熊果酸对MCF-7乳腺癌细胞生长的影响  

Effect of ursolic acid on the growth of MCF-7 breast cancer cells

作　　者：杨晓文[1] 崔明[1] 莫平[1] 

机构地区：[1]成都军区昆明总医院普外科,昆明650032

出　　处：《中华实验外科杂志》2009年第4期439-441,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察熊果酸对MCF-7乳腺癌细胞增殖、凋亡、细胞侵袭及裸鼠移植瘤生长的影响。方法将1、10、100μmol／L的熊果酸分别作用于乳腺癌MCF-7细胞12、24、48h，噻唑蓝（MTF）比色法检测细胞活力变化及生长抑制率，流式细胞术及Transwell小室法检测熊果酸作用48h时MCF-7细胞周期、细胞凋亡率及细胞侵袭力。将乳腺癌MCF-7细胞接种于裸鼠，成瘤后分为生理盐水对照组、熊果酸低剂量组（每日1mg／kg）、中剂量组（每日5mg／kg）和高剂量组（每日25mg／kg），检测5、10、15d裸鼠移植瘤体积、肿瘤生长抑制率及15d时肝肾功能、血常规变化。结果随熊果酸浓度增加及作用时间延长，MCF-7细胞生长及凋亡发生受到显著影响，100μmol／L熊果酸作用48h时，细胞生长抑制率为46．0％，生长周期阻滞于G0／G1期，细胞凋亡率为（16．48±2．46）％，细胞侵袭力显著下降。熊果酸组裸鼠移植瘤体积显著小于对照组，15d时熊果酸高剂量组肿瘤体积为（323．5±33．5）mm^3，生长抑制率为50．9％。各组肝肾功能和血常规无明显变化。结论熊果酸可引起体外乳腺癌MCF-7细胞增殖抑制、细胞凋亡率增加及细胞侵袭力下降，可显著抑制裸鼠乳腺痛移槽瘤牛长。Objective To investigate the effect of ursolic acid on proliferation,apoptosis,invasion of MCF-7 breast cancer cells and MCF-7 breast tumor growth in nude mice. Methods In the ursolic acid group, MCF-7 cells were treated with ursolic acid at concentrations of 1,10 ,or 100 μmol/L for 12,24 and 48 h respectively. Cell viability of MCF-7 cells and growth inhibition rate were assayed by MTT methods. Cell cycle, apoptosis rate and cell invasiveness were assayed by flow cytometry and transwell chamber methods. Nude mice transplanted with MCF-7 cells were divided into saline control group ,low-dose ursolic acid group （ 1 mg/kg · d^ - 1 ）, middle-dose ursolic acid group （ 5 mg/kg· d^ - 1 ） and high-dose ursolic acid group （25 mg/kg· d^ - 1 ）. Tumor size, and tumor growth inhibition rate on the day 5,10 and 15 after transplantation in the nude mice were detected. Chnages in liver and renal function, and routine blood at 15th days were tested. Results With the increase of ursolic acid concentration and the prolongation of action time, MCF-7 cell growth and apoptosis were affected significantly. After MCF-7 cells were treated with 100 μmol/L ursolic acid for 48 h, the cell growth inhibition rate was 46.0% , the growth cycle was arrested in G0/G1 phase, the apoptosis rate was （16.48 ± 2.46）%, and the cell invasiveness decreased significantly. Tumor size in the ursolic acid group was significantly smaller than in the control group. In the high-dose ursolic acid group at 15th day, the tumor size was （323.5 ± 33.5 ） mm^3 , the tumor growth inhibit rate was 50.9% , and the liver and renal function, routine blood had no significant changes. Condusion Ursolic acid can inhibit proliferation and invasiveness of MCF-7 breast cancer cells, and induce apoptosis rate in vitro. Ursolic acid can significantly inhibit the growth of breast cancer in nude mice.

关 键 词：乳腺癌 熊果酸 增殖 

分 类 号：R737.9[医药卫生—肿瘤] R735.7[医药卫生—临床医学]