小剂量表柔比星对乳腺癌细胞Ezrin表达和迁移的抑制作用  

Inhibition of the expression of Ezrin and cell migration of breast cancer cells by low dose of epirubicin

作　　者：马力[1,2] 刘月平[3] 张祥宏[1] 邢凌霄[1] 王俊灵[1] 耿翠芝[2] 

机构地区：[1]河北医科大学基础研究所病理实验室,石家庄050017 [2]河北医科大学第四医院外一科,石家庄050011 [3]河北医科大学第四医院病理科,石家庄050011

出　　处：《肿瘤》2009年第3期215-219,共5页Tumor

基　　金：河北省普通高校强势特色学科肿瘤学建设资助项目(编号:200552)

摘　　要：目的:探讨小剂量表柔比星(epirubicin,EPI)对人乳腺癌细胞MDA-MB-231中Ezrin表达和迁移能力的影响,以期为临床合理用药提供新的思路。方法:应用RT-PCR、Western印迹法和免疫细胞化学方法分别从基因、蛋白和细胞水平检测在小剂量EPI作用下,人乳腺癌细胞MDA-MB-231中Ezrin的表达水平及细胞迁移能力的变化,以及Ezrin与E-cadherin和CD44表达的相关性。结果:在不同浓度(1.0、2.5、5.0、10.0μg/mL)EPI作用4h后,乳腺癌细胞的迁移能力受到明显抑制。小剂量EPI以剂量依赖方式从蛋白和基因2个水平抑制乳腺癌细胞中Ezrin和CD44的表达,并且以剂量依赖性方式提高E-cadherin的表达。结论:小剂量EPI可以通过抑制Ezrin的表达,进而降低乳腺癌细胞的迁移能力,这一作用并不依赖其特有的细胞毒性。Objective：To investigate the effect of low dose of epirubicin （EPI） on Ezrin expression and cell migration of human breast cancer MDA-MB-231 cells and provide a new idea for rational drug usage in clinic. Methods：RT-PCR, Western blotting, and immunocytochemistry were used to detect the Ezrin expression in human breast cancer MDA-MB-231 cells at mRNA, protein, and cellular levels, respectively, after low dose of EPI treatment. Migration abilities of breast cancer cells were observed after low dose of EPI treatment. The correlation of Ezrin with the expression of E-cadherin and CD44 was analyzed. Results：The migration capabilities of breast cancer cells were inhibited significantly after 4-h treatment with EPI at 1.0, 2.5, 5.0, and 10.0 μg/mL. Low dose of EPI inhi-bites the mRNA and protein expressions of Ezrin and CD44 and elevated the expression of E-cadherin in a dose-dependent manner. Conclusion： Low dose of EPI decreases the migration ability of breast cancer cells via inhibiting Ezrin expression. The effect is not dependent on its specific cytotoxicity.

关 键 词：乳腺肿瘤 抗肿瘤联合化疗方案 表柔比星 细胞运动 

分 类 号：R737.9[医药卫生—肿瘤] R73-361[医药卫生—临床医学]