骨保护素对犬牙齿萌出过程中方组织内破骨细胞的影响  

Effects of osteoprotegerin on osteoclasts in the occlusal tissue during tooth eruption in dog

作　　者：陈丽萍 吴炜 

机构地区：[1]西宁武警青海总队医院口腔科,810001

出　　处：《实用口腔医学杂志》2009年第2期197-200,共4页Journal of Practical Stomatology

摘　　要：目的:在体研究骨保护素对正常犬牙齿萌出过程中方组织内破骨细胞的影响,并分析其机制。方法:同一窝6只出生7d本地犬随机分成对照组和骨保护素组,后者皮内注射骨保护素每日1.5mg/kg,连续3d。自注药起5d取其下颌骨,制备石蜡切片,分别用酶组织化学和免疫组织化学的方法检测犬下颌第三前磨牙牙胚方组织中破骨细胞、RANKL阳性细胞的变化情况。结果:骨保护素组中平均破骨细胞数、RANKL表达较对照组明显降低(P<0.05)。结论:骨保护素对正常犬牙齿萌出过程中方组织内的破骨细胞可能有抑制作用,这可为牙齿萌出的研究提供一定参考。Objective：To observe the effects of osteoprotegerin on osteoclasts in the occlusal tissue during tooth eruption in dog and analyze the mechanism. Methods ： Six 7- day- old brood dogs were divided randomly to control and osteoprotegerin groups. The dogs in osteoprotegerin group were injected with osteoprotegerin for 3 days [ 1.5 mg/ （ kg · d） . 5 days after injection the dogs were sacrificed and mandible were taken out for paraffin slice. The number of the osteoclasts in the occlusal tissue up the third mandibular premolar was detected by tartrate-resistance acid phosphatase（TRAP） staining. Immunohistochemistry was used to examine the expressions of RANKL. Results： The number of osteoclasts in osteoprotegerin group was less than that in the control group （ P 〈 0.05 ）. There was significant difference for RANKL protein between osteoprotegerin and the control groups （ P 〈 0.05 ）. Conclusion ： Osteoprotegerin probably inhibit osteoclast in the occlusal tissue during tooth eruption in dog, which may provide reference to investigation about tooth eruption.

关 键 词：骨保护素 牙齿萌出 破骨细胞 RANKL 

分 类 号：R780.2[医药卫生—口腔医学]