口腔鳞状细胞癌中P27和增殖细胞核抗原的表达  

Expressions of P27 protein and PCNA in oral squamous cell carcinomas

作　　者：王雪[1] 张茹慧[1] 

机构地区：[1]长春吉林大学口腔医学院口腔颌面外科,130041

出　　处：《实用口腔医学杂志》2009年第2期256-260,共5页Journal of Practical Stomatology

摘　　要：目的:探讨P27和增殖细胞核抗原(PCNA)在口腔鳞状细胞癌(OSCC)中的表达、两指数间的关系以及临床意义。方法:应用免疫组化SP法检测OSCC55例、正常口腔黏膜上皮组织5例以及异常增生口腔黏膜上皮20例的P27蛋白和PCNA的表达。结果:P27蛋白及PCNA在OSCC中的阳性表达率分别为40%,80%,与正常及增生的口腔黏膜上皮相比均有显著差异(P<0.01,P<0.05);两者均与性别、年龄无关(P>0.05);均与临床分期、分化程度、淋巴结是否转移各自相关(P<0.05);两者在OSCC中表达呈负相关(r=-0.510,P<0.01),在异常增生的口腔黏膜中表达为不相关(r=0.154,P>0.05)。结论:P27表达的减弱和PCNA表达增强可以各自成为判定OSCC的恶性程度及预后的指标,深入研究两者的相关关系对OSCC的临床诊断和治疗均有着极为重要的价值。Objective： To investigate the expressions of P27 protein and proliferating cell nuclear antigen（PCNA） in oral squamous cell carcinoma（OSCC） and to determine the relationship between them. Methods： SP immunohistochemistry was used to detect P27 protein and PCNA in 55 cases of OSCC, 5 biopsies of normal oral mucosa and 20 hyperplastic tissues. Results： The expression rate of P27 protein and PCNA in OSCC were 40.0%, 80.0%, which were different from normal oral mucosa and hyperplasia tissues（P 〈 0.01 ,P 〈0. 05 ）. There was no correlation with age and gender（ P 〉 0.05 ）. They correlated with malignant behaviors, clinical stages and whether there was lymphnode metastasis or not of the neoplasm（ P 〈 0. 05 ）. There were an inverse correlations between the expressions of P27 protein and PCNA in OSCC （ r = - 0. 510 , P 〈 0.01 ）, but not in hyperplasia tissues （ r = 0. 154, P 〉 0.05 ）. Conclusion： Decrease of P27 protein expression and increase of PCNA expression may be important indicators which stand for malignant behavior of neoplasm. The results suggest that their inverse correlation between PCNA and P27 in various forms of oral squamous cell carcinomas may be of prognostic and therapy value.

关 键 词：P27 蛋白 PCNA OSCC 免疫组化 

分 类 号：R739.8[医药卫生—肿瘤]