NT-3对腺样囊性癌细胞系ACC-M体外侵袭能力的影响  

Effects of neurotrophin-3 on in vitro invasion of adenoid cystic carcinoma cell line ACC-M

作　　者：孙沫逸[1] 李志进[1] 李建虎[1] 王磊[1] 余立强[1] 

机构地区：[1]西安第四军医大学口腔医学院口腔颌面外科,710032

出　　处：《实用口腔医学杂志》2009年第2期261-263,共3页Journal of Practical Stomatology

基　　金：国家自然科学基金项目(编号:30271424;30772428);陕西省自然科学基金项目(编号:2002C2-06)

摘　　要：目的:研究NT-3对人涎腺腺样囊性癌细胞系ACC-M体外侵袭能力的影响。方法:应用Transwell培养小室测定不同浓度NT-3对ACC-M细胞的体外侵袭能力的影响。结果:ACC-M细胞与2.5、5、10ng/ml浓度的NT-3共培养20h后侵袭细胞数均明显多于未加NT-3组;而与浓度为100ng/ml的NT-3共培养20h后侵袭细胞数却明显减少。结论:NT-3对ACC-M细胞系体外侵袭能力的影响存在浓度依赖性。一定浓度的NT-3能够增强ACC-M细胞系的体外侵袭能力。Objective：To observe the effects of neurotrophin-3 on invasion of adenoid cystic carcinoma cell line ACC- M in vitro. Methods： Transwell cell culture chamber was applied to study the effects of different concentrations NT-3 on invasion of ACC-M cells in vitro. Results： The number of invading cells incubated for 20 h with NT-3 of 2.5, 5, 10 ng/ml was higher than cells without NT-3. But when incubated with NT-3 of 100 ng/ml for 20 h, the number of invading cells significantly decreased. Conclusion： The effects of NT-3 on invasion of ACC-M cells is concentration-dependent. NT-3 can promote ACC-M cell line＇s invasion at certain concentrations in vitro.

关 键 词：神经营养素-3 腺样囊性癌 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]