水飞蓟宾对人舌癌细胞Tca的抑制作用  

Inhibition effects of silibinin on human tongue cancer cell line Tca

作　　者：李云鹏[1] 李绍青 程晓兵[1] 刘栋琪 张铁成[4] 

机构地区：[1]西安第四军医大学口腔医院颌面外科,710032 [2]口腔生物学教研室 [3]解放军第531医院口腔科 [4]西安第四军医大学口腔医院麻醉科

出　　处：《实用口腔医学杂志》2009年第2期281-284,共4页Journal of Practical Stomatology

摘　　要：目的:研究水飞蓟宾在体外对舌癌细胞的作用,并进一步探讨其可能的作用机制。方法:MTT实验测定水飞蓟宾对舌癌细胞系(Tca)的半数抑制浓度(IC50);平板克隆形成实验检测加药前后细胞的克隆形成能力;流式细胞仪检测加药前后细胞周期的变化。结果:水飞蓟宾对舌癌细胞系有较强的抑制作用,作用48h时,IC50约为100μmol/L;水飞蓟宾作用后,舌癌细胞的平板克隆形成能力降低;流式细胞仪检测结果显示细胞出现G1期阻滞。结论:水飞蓟宾在体外对舌癌细胞具有明显的抑制作用,其作用机制可能是细胞出现G1期阻滞。Objective：To study the inhibition effects of silibinin on tongue cancer cell line Tca and the possible mechanism. Methods： Tongue cancer cell line Tca was cultured to study the effects of silibinin on cell proliferation. First, MTT assay was used to evaluate the IC50 of silibinin on the cells. Second, through colony formation assay, the celt proliferation ability was detected. At last, distribution of cell cycle was analysed by flow cytometry. Results：Silibinin inhibited the growth of Tca cells. The IC50 was about 100 μmol/L, when the cells were cultured with silibinin in 48 h. Silibinin also inhibited the cells colony formation and caused G1 arrest. Conclusion： Silibinin could inhibit Tca cell proliferation ability, and the possible mechanism might be the cell cycle stopped at G1.

关 键 词：水飞蓟宾 舌癌 细胞周期 流式细胞术 

分 类 号：R739.8[医药卫生—肿瘤] Q813.11[医药卫生—临床医学]