敌百虫暴露对孕鼠血清对氧磷酶活性及胚胎发育的影响  

Effects of trichlorfon exposure on serum paraoxonase activity of pregnant mice and development of embryos

作　　者：周义军[1] 周淑芳[1] 施蓉[1] 高宇[1] 丁瑜[1] 田英[1] 

机构地区：[1]上海交通大学公共卫生学院,上海200025

出　　处：《上海交通大学学报（医学版）》2009年第3期245-247,共3页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30571542;30872086);上海市教委基金(07ZZ31);上海市科委研发基地项目(07dz22013)~~

摘　　要：目的探讨敌百虫长期低剂量暴露对孕早期血清对氧磷酶(PON)活性和胚胎发育的影响。方法选用雌性ICR小鼠120只,随机分为空白对照组和不同剂量敌百虫染毒组,每组30只,每天分别给予0、2、10、50mg/kg敌百虫进行染毒,连续染毒27d后进行交配,并对孕鼠继续染毒,于孕第3天断髓处死。测定血清PON和乙酰胆碱酯酶(AchE)活性,同时观察孕鼠胚胎的发育情况。结果血清PON活性随敌百虫染毒剂量的增加有明显下降趋势,在2、10、50mg/kg染毒组分别为(14.15±1.22)、(12.78±1.80)、(10.45±1.95)IU/mL,其中50mg/kg染毒组明显低于对照组的(13.37±2.31)IU/mL(P<0.01);10mg/kg和50mg/kg染毒组的AchE活性为(44.54±6.92)IU/mL和(41.54±4.49)IU/mL,明显低于对照组的(51.81±6.20)IU/mL(P<0.01)。各染毒组胚胎着床率和异常胚胎百分率与对照组比较差异均无统计学意义(P>0.05),但50mg/kg染毒组异常胚胎百分率较对照组有增高趋势。结论敌百虫长期低剂量暴露对孕鼠血清PON和AchE活性具有一定的抑制作用,对胚胎发育未见明显影响。Objective To investigate the effects of maternal long-term exposure to low-dose trichlorfon on the serum paraoxonase （PON） activity of pregnant mice and development of embryos. Methods Female ICR mice （n=120） were randomly divided into control group and trichlorfon groups of different doses,and were managed by intragastric injection with trichlorfon of 0,2,10 and 50mg/kg,respectively. All the mice were managed once a day for a consecutive of 27 days,and were subjected to mating. The pregnant mice were continued to be managed with trichlorfon for 3 days,and were sacrificed on day 3 of gestation. The serum PON and acetylcholinesterase （AchE） activities were detected,and the development of embryos was evaluated. Results The serum PON activity of 2,10 and 50mg/kg trichlorfon group were （14.15±1.22）,（12.78±1.80） and （10.45±1.95）IU/mL,respectively,and that of 50mg/kg trichlorfon group was significantly lower than that of control group [（13.37±2.31）IU/mL] （P〈0.01）. The serum AchE activity of 10 and 50mg/kg trichlorfon group [（44.54±6.92）IU/mL and （41.54±4.49）IU/mL,respectively] was significantly lower than that of control group [（51.81±6.20）IU/mL] （P〈0.01）. There was no significant difference in the percentage of abnormal embryos and implantation rate of embryos between trichlorfon groups and control group （P〉0.05）,while the the percentage of abnormal embryos of 50mg/kg trichlorfon group had an increased tendency. Conclusion Long-term exposure to low-dose trichlorfon can inhibit serum PON and AchE activity in pregnant mice without obvious effect on the development of embryos.

关 键 词：对氧磷酶 乙酰胆碱酯酶 敌百虫 胚胎 孕鼠 

分 类 号：R363[医药卫生—病理学]