原生质体紫外诱变选育鸟苷高产菌株  

Study on Breeding up High-yield Strain of Guanosine by Protoplast Mutagensis

作　　者：盛翠 张一平[2] 陆茂林[1,2] 

机构地区：[1]江南大学医药学院,江苏无锡214122 [2]江苏省微生物研究所有限公司,江苏无锡214063

出　　处：《食品与生物技术学报》2009年第2期279-283,共5页Journal of Food Science and Biotechnology

摘　　要：以一株鸟苷产生菌Bacillus subtilis JSIM-G518为出发菌株,采用原生质体紫外诱变的方法,选育具有核苷水解酶缺失,磺胺胍,德夸菌素、狭霉素C等抗性的突变株,摇瓶的鸟苷产量达到24.0 g/L,比出发菌株提高了30%。该突变株遗传性状稳定,连续传代10次,仍维持较高的鸟苷产量。In this study, Bacillus subtilis JSIM-G518 was used as the parent strain to breed a high guanosine production strain. Under the optimum conditions of formation and regeneration of protoplasts, the protoplasts of Bacillus subtilis JSIM-G518 were prepared. A resistant mutant strain which have lack-nucleoside hydrolyase（NHase）, sulfaguanidine （SG）, decoyinine （Dec）, psicofuranine （Psi） was obtained by UV irradiation of protoplasts. The production of guanosine was 24 g/L, was higher 30% than that of the parent strain. Further experiment confirms that Bacillus subtilis JSIM GU-124-19 is a stable strain.

关 键 词：鸟苷 枯草芽孢杆菌 原生质体 诱变育种 

分 类 号：Q933[生物学—微生物学]