bFGF对局部性脑缺血再灌注大鼠海马及皮质神经元CREB表达的影响  

Effects of bFGF on expression of CREB in hippocampus and cortex during cerebral ischemia and reperfusion in rats

作　　者：曾朝涛[1] 郭和[1] 邢雪松[1] 吕威力[2] 

机构地区：[1]沈阳医学院基础医学院解剖教研室,辽宁沈阳110034 [2]沈阳医学院基础医学院病理教研室,辽宁沈阳110034

出　　处：《第四军医大学学报》2009年第6期517-519,共3页Journal of the Fourth Military Medical University

基　　金：辽宁省教育厅科学研究计划(05L442)

摘　　要：目的:研究大鼠脑缺血再灌注后cAMP反应元件结合蛋白(CREB)表达的变化,探讨碱性成纤维细胞生长因子(bFGF)对脑组织缺血再灌注神经元CREB的调节作用及机制.方法:应用线栓法制作大鼠局灶性脑缺血再灌注模型,大脑中动脉阻塞2h再灌注损伤24h,采用TUNEL法、免疫组化检测海马及皮质内神经元凋亡和CREB的表达.结果:大鼠缺血再灌注海马及顶叶皮质内神经元凋亡数增加而CREB表达较假手术组减少,注射bFGF后神经元凋亡数减少,CREB表达明显高于缺血再灌注组.结论:bFGF抑制缺血神经元凋亡,参与CREB的调节,对缺血神经元有保护作用.AIM： To investigate the expression of cyclic AMP response element binding protein （CREB） in hippocampus and cortex after cerebral ischemia and reperfusion in rats and to explore the regulative effects of basic fibroblast growth factor （bF- GF）on CREB in brain tissue and its mechanism. METHODS： The rat models of middle cerebral arteries occlusion （MCAO） were established with intraluminal filament blockade. The expression of CREB and apoptosis of neurons in hippocampus and cortex were detected with immunohistochemistry and TUNEL method respectively. RESULTS： The expression of CREB in hippocampus and cortex decreased after cerebral ischemia and reperfusion. In bFGF group, the expression of CREB increased while cell apoptosis decreased. CONCLUSION： bFGF depresses cell apoptosis, participates in the regulation of CREB expression in ischemic neurons and protects ischemic neurons.

关 键 词：脑缺血 成纤维细胞生长因子2 CREB 海马 细胞凋亡 

分 类 号：R361.2[医药卫生—病理学]