邻苯二甲酸二(2-乙基)己酯对小鼠胎鼠睾丸及睾丸引带的影响  

Effects of Di (2-Ethylhexyl) Phthalate on the Testis and Testicular Gubernaculum of Fetal KM Mice

作　　者：宋晓峰[1] 邓永继[1] 张德迎[1] 刘星[1] 吴盛德[1] 魏光辉[1] 

机构地区：[1]重庆医科大学附属儿童医院泌尿外科,重庆400014

出　　处：《中华男科学杂志》2009年第3期195-199,共5页National Journal of Andrology

基　　金：国家自然科学基金(30371475)~~

摘　　要：目的:研究邻苯二甲酸二(2-乙基)己酯(DEHP)对小鼠胎鼠睾丸与睾丸引带形态结构及功能的影响,探讨隐睾发生的可能机制。方法:30只健康KM孕鼠随机均分为3组:空白对照组、玉米油对照组、DEHP组。DEHP组以剂量500mg/(kg.d)的DEHP灌胃作用于怀孕12～19d(GD12～GD19)的KM母鼠,观察DEHP对每胎胎鼠数、雌雄性胎鼠比例、雄性胎鼠体重、睾丸重量、睾丸引带形态结构、位置、睾丸到膀胱颈之间的相对距离(TBD)、颅侧悬韧带残留情况、引带内雄激素受体(AR)、雌激素受体(ER)、肌动蛋白、增殖细胞核抗原(PCNA)表达水平的影响。结果:DEHP对每胎胎鼠数、雌雄性胎鼠比例、雄性胎鼠体重无明显影响;DEHP可影响胎鼠睾丸重量及TBD;DEHP组睾丸均有一定程度的下降不全,睾丸引带形态结构无明显差异;光镜下见DEHP组睾丸生精小管、支持细胞存在明显的发育障碍,睾丸Leydig细胞明显增生;DEHP组睾丸引带AR阳性表达率降低(P<0.01)。结论:DEHP可通过抗雄激素效应导致睾丸引带功能障碍,使睾丸下降发生异常而诱导小鼠隐睾;同时造成睾丸Ser-toli细胞、睾丸Leydig细胞和生精细胞的发育障碍和功能改变。Objective ： To explore the effects of Di （2-ethylhexyl） phthalate （DEHP） on the testis and testicular gubernaculum of fetal KM mice in vivo and to investigate the mechanism of DEHP-induced cryptorehidism. Methods ： Thirty healthy pregnant KM mice were randomly and equally divided into a blank control group, a corn oil control group and a DEHP group. The pregnant mice in the latter group were exposed to DEHP by gavage at the dose of 500 mg/kg body weight per day from gestation day 12 （GD12） through gestation day 19 （GD19）. The effects of DEHP were observed on the number of fetuses per pregnancy, the ratio of male to female pups, the weight of the testis, the morphology and location of the testis and gnbernaculum, the relative testis-bladder neck distance （TBD） and cranial suspensory ligament （CSL） residual. The expressions of the androgen receptor （AR） , estrogen receptor （ER） and actin and proliferating cell nuclear antigen （ PCNA ） in the gubernaculums were detected by immunohistochemistry. Results ： DEHP reduced the testis weight and TBD, induced different degrees of testis maldescent, but produced no obvious effect on the body weight, the number of fetuses per pregnancy, the sex ratio and the testis gnbernacular morphology. Under the light microscope, hypotrophy was seen in all the testis seminiferous tubules, spermatogenic cells and Sertoli cells, marked Leydig cell hyperplasia was noted, and the positive expression of AR in the gubernaculum was decreased in the DEHP group （P 〈 0.01 ）. Conclusion ： DEHP could cause dysfunction of the testis gubernaculum via its anti-androgen effect, induce cryptorchidism, and cause dysplasia and dysfunction of Sertoll cells, Leydig cells and spermatogenic cells in fetal mice.

关 键 词：环境内分泌干扰物 邻苯二甲酸二(2-乙基)己酯 隐睾 抗雄激素效应 睾丸引带 胎鼠 

分 类 号：R363[医药卫生—病理学] R697.2[医药卫生—基础医学]