姜黄素对涎腺腺样囊性癌细胞系SACC-83增殖和凋亡的影响  

Effect of curcumin on proliferation and apoptosis of salivary adenoid cystic carcinoma cell line SACC-83 in vitro

作　　者：杨威[1] 陈彪[1] 张英怀[1] 贾志宇[1] 屈鹏飞[1] 郭利华[1] 张平[1] 唐全勇[1] 

机构地区：[1]河北医科大学第二医院口腔颌面外科,石家庄050000

出　　处：《现代口腔医学杂志》2009年第2期142-144,共3页Journal of Modern Stomatology

基　　金：河北省科技攻关指导项目(200527315613)

摘　　要：目的体外研究姜黄素对涎腺腺样囊性癌细胞系SACC-83增殖和凋亡的影响。方法用终浓度为2.5、5、10、15、20mg/L的姜黄素处理SACC-83。应用MTT比色试验,倒置显微镜,Giemsa染色,Annexin-V-FITC和PI双标记活细胞后流式细胞仪和透射电镜检测和观察SACC-83的生长抑制率和细胞凋亡情况。结果姜黄素对SACC-83的生长抑制率与药物浓度和作用时间具有明显的正相关性(P<0.01)。用药组细胞明显凋亡,凋亡率随处理时间延长和药物浓度增加而上升,各组间有显著差异(P<0.01)。结论姜黄素具有抑制涎腺腺样囊性癌细胞系SACC-83增殖和诱导其凋亡的作用,且具有浓度和时间依赖性。Objective To evaluate effects of curcumin on proliferation and apoptosis of salivary adenoid cystic carcinoma cell line SACC - 83 in vitro. Methods SACC - 83 cells were treated with different concentration of curcumin （2.5, 5, 10, 15, and 20mg/L respectively）. MTr assay, Giemsa staining, flow cytometry with Annexin- V- FITC/ propidiumiodide （PI） double staining, and transmission electron microscope （TEM） were used to evaluate cell growth and apoptosis. Results The growth inhibition rate of curcumin on SACC - 83 cells was positively correlated with concentrations of curcumin and treated time. The apoptosis of SACC - 83 cells was increased with concentration and treatment time of curcumin. Conclusion Curcumin could effectively inhibit proliferation and induce apoptosis of salivary gland adenoid cystic carcinoma cell line SACC -83.

关 键 词：姜黄素 腺样囊性癌 涎腺 增殖 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]