中国人甲状腺癌D2S123、D18S58、BAT-26基因的遗传不稳定性及意义  

Genetic instability and signifiance of D2S123,D18S58,BAT-26 genes in thyroid carcinoma in chinese people

作　　者：翟瑜[1] 苏力[1] 彭彦辉[1] 王跃欣[1] 杨月卿[1] 郭贵军[1] 脱洪芳[1] 

机构地区：[1]河北省人民医院,河北石家庄050051

出　　处：《山东医药》2008年第47期8-10,共3页Shandong Medical Journal

摘　　要：目的探讨中国人甲状腺癌特定位点微卫星DNA序列基因遗传不稳定性(MSI)及其临床意义。方法选取D2S123、D18S58、BAT-26三个位点,应用聚合酶链反应(PCR)和变性聚丙烯酰胺凝胶电泳技术,对30例人甲状腺癌中MSI表达情况进行研究。结果D2S123、D18S58、BAT-26位点MSI检出率分别为36.7%。甲状腺滤泡癌中D2S123位点MSI检出率为100%,没有检测到BAT-26位点MSI。D18S58位点MSI的阳性率与高龄患者、晚期肿瘤密切相关。术后随访5a,MSI阳性的甲状腺癌较阴性者生存期更长(P<0.05)。结论甲状腺癌中存在D2S123、D18S58、BAT-26位点的MSI,但检出率各不相同。上述位点MSI在甲状腺肿瘤发生过程中发挥作用,且对患者预后影响。Objective To investigate the microsatellite instability （MSI） and its signifiance in thyroid carcinoma in chinese people. Methods The polymerase chain reaction （PCR） and polyaerylamide gel electrophoresis（PAGE） technique were used to detected D2S123 .D18S58.BAT-26 MSI in 30 thyroid carcinoma tissues and normal tissues. Results In 30 thyroid carcinoma patieuts, D2S123 ,D18S58.BAT-26 loci MSI rate were 36.7% 、26.7%、.6.7%. In follicular cancer, 2S123 MSI rate was 100% and BAT-26 MSI rate was zero. Follow-up visit 5years, patients with MSI positive cancer showed better long-term survival. Conclusions There are D2S123.D18S58.BAT-26 loci MSI in thyroid carcinoma. They play an important role in genesis of thyroid carcinoma and influence its prognosis.

关 键 词：甲状腺肿瘤 甲状腺癌 基因遗传不稳定性 

分 类 号：R736.1[医药卫生—肿瘤]