组织芯片结合免疫组化检测人结肠癌组织中PTEN/p-Akt1的表达及其临床意义  

Study on Expressions and Clinical Significance of PTEN,p-Akt1 by Immunohistochemical Staining and Tissue Microarray in Human Colon Cancer

作　　者：施超[1] 陈卫昌[2] 林茂松[3] 张宝峰 方静 周琼 胡莺 郜恒骏 

机构地区：[1]启东市人民医院普外科,江苏启东226200 [2]苏州大学附属第一医院消化内科,江苏苏州215006 [3]泰州市人民医院消化内科,江苏泰州225300 [4]生物芯片上海国家工程中心,上海201203

出　　处：《苏州大学学报（医学版）》2008年第6期949-952,I0010,共5页Suzhou University Journal of Medical Science

基　　金：国家863计划功能基因组与生物芯片重大专项资助项目(2002AA2Z2021);江苏省"六大人才高峰"基金(D类)资助项目

摘　　要：目的研究结肠癌、腺瘤和相应癌旁正常结肠黏膜组织中PTEN、p-Akt1的表达及其临床意义。方法取85例结肠癌、18例结肠腺瘤和9例癌旁结肠黏膜组织分别制成72点和104点两块组织芯片,采用免疫组化法检测结肠组织芯片中PTEN、p-Akt1的表达。结果PTEN、p-Akt1在结肠癌、结肠腺瘤和正常结肠黏膜组织中的表达差异无统计学意义(P>0.05),在不同分化程度的大肠癌组织中PTEN的表达差异有统计学意义(P<0.05),与其他临床病理资料无关;p-Akt1的表达与大肠癌组织学分化程度及淋巴结转移有关(分别P<0.001和<0.05),与其他临床病理资料无关。结论PTEN、p-Akt1畸变可能未参与正常大肠细胞早期恶性转化的过程,但在结肠癌发生、发展中可能起一定作用。Objective To study the expressions and clinical significance of PTEN,p-Akt1 in colon cancer,adenomatous polyps and corresponding normal para-cancer colon mucosa.Methods Eighty-five cases of colon cancer,18 cases of adenomas and 9 cases of para-cancer mucosa were accumulated and two tissue microarrays were made which containing 72 dots and 104 dots respectively.Expressions of PTEN,p-Akt1 proteins were detected by immunohistochemical staining to these tissue microarrays.Re-sults No significant difference was found among varied tissues according to the expression of PTEN and p-Akt1（P〉0.05）.In addition,expression of PTEN has no correlation with other clinicopathological parameters but histological grades（P〈0.05）.No correlations of expression of p-Akt1 with clinicopatholog-ical data but histological grade（P〈0.001） and lymph node metastasis（P〈0.05） were found.Conclusion Though not involving in the development of colon carcinigenesis,distortion of PTEN and p-Akt1 may have potent roles in progression of colon cancer.

关 键 词：组织芯片 免疫组织化学 结肠癌 PTEN p-Akt1 

分 类 号：R735.3[医药卫生—肿瘤]