塞来昔布对N-丁基-N-（4-羟丁基）亚硝基胺所诱导的SD大鼠膀胱肿瘤环氧化酶-2表达的影响  

Influence exerted by celecoxib on the COX -2 expression of SD mice bladder tumor induced by N - butyl - N - （ 4 - hydroxybutyl） - nitrosamine

作　　者：李春[1] 邹桂华[2] 郑洁[1] 彭瑞元[1] 彭爱军[3] 邱建忠[1] 李晓铭[1] 

机构地区：[1]广东省深圳市宝安人民医院泌尿外科,518101 [2]广东省深圳市宝安人民医院病理科,518101 [3]广东省深圳市宝安人民医院动物实验室,518101

出　　处：《中国实用医刊》2009年第6期6-7,10,共3页Chinese Journal of Practical Medicine

基　　金：广东省深圳市科技局科技计划资助项目（200304257）

摘　　要：目的探讨N-丁基-N-（4-羟丁基）亚硝基胺（BBN）所诱导的膀胱肿瘤的环氧化酶-2（COX-2）的表达以及不同剂量的环氧化酶-2的抑制剂塞来昔布对BBN所诱导的SD大鼠膀胱肿瘤的COX-2的表达是否存在差异。方法本实验将SD大鼠随机分成3组，3组均以BBN为致癌剂诱发SD大鼠膀胱肿瘤的生长。同时，其中的两个实验组另外喂以不同剂量的塞来昔布（每千克饮用水中500mg塞来昔布、每千克饮用水中1000mg塞来昔布），20周停药，30周后处死动物，观察不同剂量的塞来昔布对COX-2表达的影响及COX-2的表达与膀胱癌的病理分级的关系。结果不同剂量塞来昔布对COX-2的表达未产生差异（P〉0．05）。尚不能认为COX-2的表达与膀胱肿瘤的病理分级有关（P〉0．05）。结论塞来昔布可能通过COX-2非依赖途径对BBN所诱导的SD大鼠膀胱肿瘤发挥抑制作用。Objective To detect COX -2 expression of urinary bladder tumor induced by N - butyl - N - （4 - hydroxybutyl） - nitrosamine （BBN） and whether different dosage cyclooxygenase - 2 inhibitor celecoxib prevent the SD mice urinary bladder tumor induced by BBN differently. Methods SD mice were divided into 3 groups at random, all the 3 groups were treated with BBN to induce urinary bladder cancer, meanwhile 2 of the 3 groups were fed by cetecoxib in addition at different dosage （500 mg celecoxib/kg tap water,1 000 mg celecoxib/kg tap water） , medicines were stopped after 20 weeks, the animals were killed 30 weeks later. To observe whether celecoxib exert influence on the expression of COX -2, the relationship between the expression of COX -2 and pathological grading of urinary bladder tumor. Results Different dosage of celecoxib exert no difference of COX - 2 expression （P 〉 0.05 ）, the expression of COX -2 had no relationship with the pathological grading of urinary bladder tumor （P 〉 0. 05 ）. Conclusions Perhaps by the non -COX -2 dependent way, cetecoxib exert preventive function on urinary bladder cancer induced by BBN.

关 键 词：环加氧酶抑制剂/药理学 膀胱肿瘤 丁基羟丁基亚硝基胺 

分 类 号：R737[医药卫生—肿瘤]