孕期暴露于脂多糖对子代中年大鼠血管重构和血压的影响及其机制  

Alteration of vascular remolding and blood pressure in rat offsprings following maternal exposure to LPS during pregnancy

作　　者：苗莹[1] 唐渊[1] 李晓辉[1] 

机构地区：[1]第三军医大学新药研究中心暨药剂学教研室,重庆400038

出　　处：《第三军医大学学报》2009年第7期569-571,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30672641);国家高技术研究发展计划(863计划)(2007AA10Z356)~~

摘　　要：目的探讨孕期炎症刺激对子代中年大鼠的血管重构及血压的影响。方法SD孕鼠分2组:脂多糖(LPS)组在孕期第8、10、12天腹腔注射LPS0.79mg/kg;对照组在同时期腹腔注射等体积生理盐水。子代大鼠出生后按随机数字表法分别选取9只,分做2组进行实验。子代大鼠第35周,取主动脉做病理切片后测量中层壁厚(MT)、管腔内径(LD),得MT/LD值,ELISA法检测血管平滑肌细胞的PCNA表达量,硝酸还原酶法测血清一氧化氮(NO)浓度,放免法测血浆内皮素-1(ET-1)浓度,无创血压仪测量鼠尾动脉收缩压。结果孕期暴露于LPS致子代大鼠主动脉MT/LD值明显增大,血管平滑肌细胞增殖,血浆ET-1明显升高,尾动脉收缩压明显增高。结论孕期暴露于炎症刺激剂可导致子代中年大鼠血管重构,血管功能紊乱,血压升高。Objective To explore the effect pressure in mid-aged rats. Methods Time-mated of prenatal inflammation on vascular remolding and blood pregnant Sprague-Dawley （SD） rats were randomly divided into 2 groups, received peritoneal injection of 0.79 mg/kg LPS on the gestation days 8, 10, and 12, or same volume of the sterile saline at the same time points. Nine pups were randomly selected from each group for the later experiments, and the offspring were named as LPS groups for those having prenatal LPS exposure and con- trol group for those having not. Their blood pressure was determined with a rat tail non-invasive instrument by tail-cuff method from 6 weeks old to 35 weeks old, and then their aortas were taken out for media thickness （MT）, diameter of lumen （LD）, and the ratios of MT/LD. The expression of proliferating cell nuclear antigen （PCNA） on the vessel was detected by ELISA. The serum level of NO and plasma endothelin-1 （ ET-1 ） were measured by nitrate reduetase and radioimmunoassay respectively. Results Compared with control group, LPS groups had significantly raised blood pressure, a significantly higher ratio of MT/LD, obviously increased expression of PCNA, and markedly elevated serum ET-1. Conclusion The offspring whose prenatal rats were exposured to LPS result in vascular remolding, vasofunctional disturbances and hypertension.

关 键 词：炎症 LPS 高血压 血管重构 

分 类 号：R361.2[医药卫生—病理学] R364.5[医药卫生—基础医学]