吸烟与肺组织DNA加合物形成关系的研究  

Studies on the Relationship between Smoking and Formation of DNA Adducts in Lung Tissues

作　　者：吕京[1,2] 杨柯[1,2] 崔涛 谢汇江[1,2] 张海青 孟庆海 赵志文[1,2] 贺锡雯[1,2] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所 [2]北京市结核病胸部肿瘤研究所

出　　处：《中华预防医学杂志》1998年第3期145-146,共2页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金

摘　　要：目的探讨吸烟与人肺组织ＤＮＡ加合物含量的关系以及在肺癌患者与非肺癌患者之间、男女性之间有无差别。方法提取同期住院原发肺癌患者与非肺癌患者（男各１９例，女各１８例）手术切除正常肺组织ＤＮＡ，采用３２Ｐ后标记方法测定ＤＮＡ加合物含量。结果吸烟可明显地引起肺组织ＤＮＡ加合物含量增高。如果控制吸烟因素，男女之间，非肺癌患者与肺癌患者之间的肺组织ＤＮＡ加合物水平差异无统计学意义。Objective To explore the relationship between smoking and contents of DNA adducts in the lung tissues, and to study if there is any its difference between the patients with and without lung cancer and between males and females. Methods DNA was extracted from the normal lung tissues dissected from the concurrently hospitalized patients with and without lung cancer (19 and 18 each for males and females). Contents of DNA adducts in the lung tissues were determined by 32 P postlabelling assay. Results Smoking could cause increase in levels of DNA adducts in the lung tissues. There were no significant difference in DNA adduct levels between the lung tissues from men and women and from the patients with and without lung cancer, after the adjustment for smoking factor. Conclusion More data should be accumulated to elucidate the relationship between DNA adduct levels in the human bodies and its biological effects.

关 键 词：肺肿瘤 DNA加合物 吸烟 同位素标记 

分 类 号：R734.202[医药卫生—肿瘤]