外周血单个核细胞在不同pH条件下HSP70形成的研究  

作　　者：刘敬波[1] 刘世文[1] 王连文[1] 王树云[1] 陈佳荣[1] 

机构地区：[1]滨州医学院附属医院,山东滨州256603

出　　处：《中外医疗》2008年第29期3-3,5,共2页China & Foreign Medical Treatment

摘　　要：目的探讨不同pH血清对热休克蛋白形成的影响。方法抽取16例健康志愿者外周血,应用密度梯度法分离单个核细胞,分别在不同pH血清中42℃热休克0.5、1h,用免疫组化测定热休克蛋白的形成。结果体外血单个核细胞在不同pH血清中热休克蛋白形成有明显差异。结论热休克蛋白的形成速度与应激源数量密切相关。Objective To determine whether different pH serum has effect on the formatinon of HSP70.Methods Whole blood was taken from 16 healthy volunteers,PBMC were separated by Percoll density gradient centrifugation,tben hot shocked in different pH serum under 42℃,0.5 and 1 hour,HSP70 was detected by immunohistochemical staining.Results Compare with blank,the expresson of HSP70 on PBMC in different pH serum were significantly difference.Conclusion The expressons of HSP70 on PBMC was closely related to the number of stress source.

关 键 词：单个核细胞 热休克蛋白 免疫组化染色 

分 类 号：R73-3[医药卫生—肿瘤]