小鼠T淋巴细胞白血病细胞系(L615)B7瘤苗抗肿瘤免疫的实验研究  

Experimental study of antitumor immunity induced by B7 vaccine of a highly malignant murine leukemic T cell line(L615)

作　　者：李牧[1] 尤胜国[1] 廖晓龙[1] 魏代奎 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所

出　　处：《中华血液学杂志》1998年第5期230-233,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金;天津市自然科学基金

摘　　要：目的：探讨高度恶性的小鼠Ｔ淋巴细胞白血病细胞系Ｌ６１５转导Ｂ７１基因后激发移植宿主体内的抗肿瘤免疫情况。方法：以逆转录病毒为载体，将Ｂ７１基因导入小鼠白血病细胞系Ｌ６１５中，获得高表达Ｂ７１分子的Ｌ６１５Ｂ７细胞，作为肿瘤疫苗进行体内移植，观察其免疫保护作用并检测瘤苗体外激活的Ｔ细胞杀伤活性、细胞增殖和因子分泌情况。结果：体内移植实验表明Ｂ７１分子的表达可降低Ｌ６１５细胞在小鼠中的致瘤性，明显延长其生存时间。用Ｂ７瘤苗预先免疫小鼠，对随后的瘤细胞攻击有明显的免疫保护作用。Ｂ７瘤苗体外活化的Ｔ细胞对同种细胞有特异性杀伤活性，并可刺激Ｌ６１５Ｂ７的增殖。结论：Ｂ７１基因的导入并有效表达可增强肿瘤细胞免疫原性，激活Ｔ细胞。Objective: To assess the potential of B71 vaccine in inducing immunity to leukemic cells. Methods: B71 gene was introduced into L615 cells and then the positive clone (L615B7) highly expressing B71 was selected. Tumorigenic and immunoprotective activities of L615B7 cells were studied in vivo. T cell functions of cytotoxicity , proliferation and growth factor secretion were detected in vitro.Results: Syngeneic 615 mice receiving L615B7 cells survived longer than control. After being immunized with L615B7,the mice gained remarkable immunoprotection against the following challenge of wildtype L615 cells, and in some mice could survive over a long duration. In vitro, the B7activated effector cells exhibited a specific cytotoxicity to L615 and L615B7 cells,and an enhanced proliferation in response to L615B7. Conclusion:B7 vaccine could improve the immunogenicity of tumor cells , activate T cells and enhance in vivo the antitumor immunity.

关 键 词：白血病 T细胞白血病 肿瘤免疫 瘤苗 B7-1 

分 类 号：R733.03[医药卫生—肿瘤]