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作 者:曾辉[1,2] 赵相印[1,2] 马雅銮 赵如冰[1,2] 杨敏 张勇[1,2] 刘复强[1,2] 高天祥[1,2]
机构地区:[1]首都医科大学附属北京同仁医院 [2]首都医科大学实验中心
出 处:《中华血液学杂志》1998年第5期237-240,共4页Chinese Journal of Hematology
基 金:北京市自然科学基金;Roche亚洲基金
摘 要:目的:探讨全反式维甲酸(ATRA)和9顺式维甲酸(9cisRA)对HL60细胞Fas表达水平及维甲酸对抗Fas抗体诱导的细胞凋亡敏感性的影响。方法:用流式细胞仪检测HL60细胞膜Fas蛋白表达水平;分别采用形态学观察、DNA电泳和流式细胞仪检测抗Fas抗体诱导的细胞凋亡。结果:HL60细胞Fas弱表达。经10-6mol/LATRA或9cisRA分别预处理4天后,HL60细胞Fas表达水平及对抗Fas抗体诱导凋亡的敏感性均显著提高。9cisRA提高HL60细胞对抗Fas抗体诱导凋亡的敏感性的能力强于ATRA。结论:维甲酸可上调HL60细胞表达Fas,这可能与维甲酸诱导HL60细胞分化相关。进一步深入探讨其分子机制,将为肿瘤治疗。Objective: To investigate the effect of alltrans retinoic acid (ATRA) and 9cis retinoic acid (9cis RA) on the expression of Fas and the sensitivity to antiFas induced apotosis in HL-60 cells. Methods: Fas expression was detected by flow cytometry. Anti-Fas induced apoptosis (AFIA) was determined by morphological features, DNA fragment electrophoresis, and flow cytometric cell cycle analysis. Results: Fas antigen was weakly expressed on HL-60 cells. After incubation of HL-60 cells with 10^-6mol/L ATRA or 9cis RA for 4 days, both the Fas expression and AFIA were significantly enhanced. 9cis RA was more potent than ATRA in increasing the sensitivity to AFIA. Conclusion: RA might upregulate Fas expression. Further elucidation of its molecular mechanisms might provide a rationale for new therapeutic strategies of malignant diseases.
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