人rIL-2对鼠脾细胞杀瘤功能的影响  

Effect of human rIL2 on tumor cytotoxicity murine splenic cells DENG SongHua, WANG SiYing, CHENG Jie, ZHANG BaoTing,

作　　者：邓松华[1,2] 汪思应[1,2] 程洁[1,2] 张宝霆 姚立人[1,2] 任伟 王丹[1,2] 

机构地区：[1]安徽医科大学病理生理学教研室 [2]安徽省立医院肾内科

出　　处：《中国药理学通报》1998年第2期143-144,共2页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金

摘　　要：目的探讨ｒＩＬ－２抗肿瘤机制。方法用“ＬＤＨ释放法”检测经不同处理的脾细胞对其敏感的靶细胞ＹＡＣ－１，不敏感的ｌｅｗｉｓ肺癌（ＬＬＣ）及耐受的ＬＬＣ肺转移细胞（ＬＬＣＦ１）体外杀伤功能。结果正常脾细胞对３种靶细胞的杀伤能力有明显差异（Ｐ＜０．０１）；经人ｒＩＬ－２体外激活后（ＬＡＫ细胞），杀瘤能力明显增强，并对ＬＬＣ、ＬＬＣＦ１细胞的杀伤作用已无明显差异；对ＬＬＣ血道转移也有明显抑制作用；激活时间对脾细胞杀瘤能力也有影响。结论人ｒＩＬ－２能激活小鼠脾细胞，使其杀瘤尤其是对具有抗ＮＫ细胞的肿瘤杀伤能力明显增强，对肿瘤转移也有疗效。AIM To study the antitumor mechnism of human rIL2. METHODS The cytotoxicity against YAC1, LLC, LLCF1 cell of murine splenic cell treated differently is assayed by “lactic acid dehydrogense release” method. RESULTS The cytotoxicities of normal murine splenic cells against three target cells are significantly variant (P<001). After being activated by human rIL2 in vitro (as LAK cell), its antitumor activity increased, especially to LLCF1 cells. Being activated in different time, the cytolysis of LAK cells against targets is various. LAK cells can inhibit the LLC murine experimental pulmonary metastasis in vivo. CONCLUSION These results indicated the LLC murine splenic cells can be activated by human rIL2, and antitumor activity increases, especially to the tumor cell against NK cells. LAK cells are found to be effective in the therapy of LLC lung metastases.

关 键 词：RIL-2 LAK细胞 肿瘤转移 肿瘤 生物疗法 

分 类 号：R730.5[医药卫生—肿瘤]