狂犬病病毒SRV_9株N、P、G和L蛋白基因的克隆及表达载体构建  

Cloning and Vector Construction of N,P,G and L Gene from Rabies Virus Oral Vaccine Strain SRV_9

作　　者：魏玉荣[1] 易忠[1] 符子华[1] 马素贞[2] 王晓萍[3] 简子健[2] 魏婕[1,2] 王海烽[1,2] 朱晶晶[4] 

机构地区：[1]新疆畜牧科学院兽医研究所,乌鲁木齐830000 [2]新疆农业大学动物医学学院,乌鲁木齐830052 [3]新疆农业大学教育学院,乌鲁木齐830052 [4]塔里木大学,新疆阿拉尔843300

出　　处：《新疆农业科学》2009年第2期354-358,共5页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区高技术研究发展计划项目(200611107)

摘　　要：以狂犬病病毒RNA为模板,采用RT-PCR方法克隆获取N、P、G及L蛋白编码基因核苷酸序列,应用定向克隆技术,构建真核表达载体pcDNA3.1-N、pcDNA3.1-P、pcDNA3.1-G及pcDNA3.1-L。经测序鉴定证实获取的N、P、G及L蛋白区全长核苷酸序列与文献报道的一致,并成功构建了其真核表达载体。Using the total RNA isolated from rabies virus as a template, the cDNA encoding N, P, G and L was amplified by reverse transcription polymerase chain reaction method. Further, the PCR product was cloned into pGEM - T Easy and sequenced. The cDNA was verified identicaly with related literatures by sequenced, and the recombinant vectors are constructed successfully.

关 键 词：狂犬病病毒SRV9 RT—PCR 基因序列分析 表达载体构建 

分 类 号：S851.655[农业科学—预防兽医学]