饲料中羊源组织成分的PCR检测  

Species-Specific PCR Assay for Detection of Ovine-Derived Materials in Feedstuff

作　　者：徐艳芳[1] 琚春梅[1] 陈立军[1] 赵明秋[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《华南农业大学学报》2009年第2期90-93,共4页Journal of South China Agricultural University

基　　金：广东省科技计划项目(2003B21408)

摘　　要：应用Chelex-100快速提取动物基因组DNA的方法,并根据羊线粒体基因的核苷酸序列设计1对引物,通过对分离的DNA进行PCR扩增、基因克隆及核苷酸序列分析,实现了对动物源性饲料中羊组织成分的检测.结果显示,该方法对羊组织DNA检测的敏感性可达20.16 pgμ/L,当肉骨粉样品中含有质量分数为0.050%的羊源性成分时仍能检出,整个过程约需3 h.A method was developed for the detection of ovine-derived materials in imported feedstuff, in which Chelex-100 as matrix for fast extraction of ovine genomic DNA was adopted and a pair of primers designed on the sequence of ovine mitochondrial DNA were used for PCR amplification. The amplified specific fragment of the ovine mtDNA was demonstrated by both cloning and sequencing. This method allows detection of the presence of ovine mtDNA in feedstuffs containing 20. 16 pg/μL of genome DNA; 0. 050% （w） of ovine meat, and the whole process takes only 3 h.

关 键 词：PCR检测 饲料 羊源成分 

分 类 号：S816.48[农业科学—饲料科学]