食管癌患者化疗前后CD4＋CD25high调节性T细胞及Foxp3mRNA的表达及其临床意义  

The expression and clinical significance of CD4^+ CD25^(high) regulatory T cells and Foxp3 mRNA in advanced esophageal cancer patients pre-treated and treated with chemotheraphy

作　　者：段巧虹[1] 王刚[1] 罗庆礼[2] 季楚舒[1] 柯丽红[3] 范平生[3] 胡冰[1] 

机构地区：[1]安徽医科大学附属省立医院肿瘤内科,合肥230022 [2]安徽医科大学病原生物学教研室,230032 [3]安徽省肿瘤医院肿瘤内科,233004

出　　处：《临床肿瘤学杂志》2009年第3期196-198,共3页Chinese Clinical Oncology

基　　金：安徽省科技厅重点科研计划资助项目(06023090C);安徽省自然科学基金资助项目(007413256X)

摘　　要：目的:探讨晚期食管癌患者化疗前后外周血中CD4+ CD25high调节性T细胞及Foxp3 mRNA的表达变化及其临床意义。方法:采用流式细胞术检测68例晚期食管癌患者化疗前后外周血CD4+ CD25high调节性T细胞的水平,并与40例健康成人进行比较。同时运用RT-PCR方法检测其中40例患者化疗前后外周血调节性T细胞Foxp3基因mRNA的表达情况。结果:(1)食管癌患者外周血中CD4+ CD25high调节性T细胞占CD4+T细胞总数的(1.82±0.54)%,显著高于健康对照组的(1.52±0.70)%(P<0.01);(2)化疗前患者外周血中CD4+ CD25high调节性T细胞为(1.82±0.54)%,明显高于化疗后的(1.66±0.58)%(P<0.05);(3)化疗前患者调节性T细胞Foxp3基因mRNA的相对表达量为0.318±0.027,明显高于化疗后的0.266±0.028(P<0.05)。结论:食管癌患者接受化疗后外周血中Foxp3 mRNA水平与CD4+ CD25high调节性T细胞数量表达均显著下降,推测Foxp3基因可能对CD4+ CD25highT细胞有重要的调节功能,从而影响食管癌患者化疗后CD4+ CD25high调节性T细胞的水平。Objective：To explore the changes of CD4＋CD25high regulatory T cells and Foxp3 mRNA in patients with advanced esophageal cancer and its clinical significance. Methods：The frequency of CD4＋CD25high regulatory T cells in peripheral blood of 68 patients with advanced esophageal cancer and 40 healthy controls was determined by flow cytometry, the expression of Foxp3 mRNA in these regulatory T cells of 40 patients with advanced esophageal cancer were detected by RT-PCR. Results：CD4＋CD25high regulatory T ceils numbers were significantly more in 68 patients with advanced esophageal cancer （ CD4＋ T cells） than in 40 healthy donors. The level of CD4＋CD25high T cells was more in advanced esophageal cancer patients treated with chemotheraphy than in those patients with- out chemotheraphy. The level of Foxp3 mRNA were significantly less in advanced esophageal cancer patients treated with chemotheraphy than in those patients without chemotheraphy. Conclusion：As the level of CD4＋CD25highT cells, the level of Foxp3 mRNA significantly droped in advanced esophageal cancer patients treated with chemotheraphy, and Foxp3 genes may play a pivotal role in regulating the function of Treg cells and the level of CD4＋CD25highT in advanced esophageal cancer patients treated with chemotheraphy.

关 键 词：晚期食管癌 CD4+CD25high调节性T细胞 FOXP3 

分 类 号：R735.1[医药卫生—肿瘤]