基于膨润土的层柱黏土固定β-葡萄糖醛酸苷酶的研究  

Immobilization of β-glucuronidase on pillared interlayer clays from bentonite

作　　者：党艳艳[1] 冯世江[1] 栗全营[1] 李春[1,2] 

机构地区：[1]石河子大学食品学院新疆兵团化工绿色过程重点实验室,石河子832003 [2]北京理工大学生命科学与技术学院,北京100081

出　　处：《生物加工过程》2009年第2期44-47,共4页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(20776017);北京市自然科学基金资助项目(5072028);石河子大学高层次人才科研启动项目(RCZX200629)

摘　　要：以膨润土制备的层柱黏土为载体,考察给酶量、固定化pH、温度和时间对固定化β-葡萄糖醛酸苷酶活性的影响,并对其操作稳定性进行研究。结果表明:给酶量为2 700 U/g,最适pH为3.6,固定化温度40℃,固定化60 m in条件下固定化酶催化活性较高;酶经固定化后其热稳定性及储存稳定性显著提高。Immobilization of β-glucuronidase on pillared interlayer clays （PILC） from bentonite was studied. The factors related with the activity of the immobilized β-glucuronidase on PILC, including the amount of β-glucuronidase,pH,temperature, and time of the immobilization reaction, were investigated. The results showed that the optimum conditions for immobilization were as follows ：β-glucuronidase dosage was 2 700 U/g carrier, immobilizing for 60 min at 40℃ at pH 3.6. The temperature and the storage stability of the enzyme were obviously increased after the treatment.

关 键 词：层柱黏土 Β-葡萄糖醛酸苷酶 甘草酸 固定化 

分 类 号：TQ925[轻工技术与工程—发酵工程]