胃癌前病变中Bcl-2、Bax表达及与Hp感染的关系  

Relationship between Helicobacter pylori infection and Bcl-2,Bax expression in precancerous lesions

作　　者：张明华[1] 代二庆[1] 袁红霞[2] 赵占考[1] 景秀荣[1] 张磊[1] 孙捍卫[1] 

机构地区：[1]武警医学院附属医院检验科,天津300162 [2]天津中医药大学文理部,天津300192

出　　处：《武警医学》2009年第3期232-234,共3页Medical Journal of the Chinese People's Armed Police Force

基　　金：国家自然科学基金面上项目(30371708);天津市科委应用基础研究面上项目(05YFJMJC08400)

摘　　要：目的探讨慢性萎缩性胃炎癌前病变不同病理改变,胃上皮细胞的凋亡相关基因蛋白表达及与幽门螺杆菌（Hp）感染的相关性。方法应用快速尿素酶试验与W-S银染色法将各种病变分为Hp感染阳性组和阴性组,应用免疫组化SP法对各种病变凋亡相关基因Bcl-2、Bax的表达进行检测。结果重度肠化生组的Bcl-2基因蛋白表达非常显著优于轻度组、中度组（P〈0.01）。从总体上看Hp阳性组Bax、Bcl-2的表达与Hp阴性组无显著差异,但各组别中,中度组,Hp阳性组Bcl-2的表达明显低于Hp阴性组（P〈0.01） 重度组,Hp阳性组Bcl-2、Bax的表达明显高于Hp阴性组（P〈0.01）。结论癌前病变病理程度的变化与Bcl-2、Bax基因蛋白表达变化有关,Hp的感染可以影响两者的表达,这种变化可能是Hp相关性慢性萎缩性胃炎癌前病变细胞凋亡变化的原因之一。Objective To investigate wether Helicobacter priori infection has any effects on Bcl -2 and Bax gene associated apoptosis, as well as its role in the different pathology of chronic atrophic gastritis accompanied by precancerous lesion. Methods Helicobacter pylori infection was determined by microscopic examina- tion , by Giemsa staining and rapid urease test. S - P immunohisto- chemical analysis was used to detect the expression of Bcl - 2 and Bax in precancerous lesions caused by H. pylori and other causes. Results The positive rate of Bcl - 2 protein in the sever intestinal meta- plasia group was significantly higher than that in the mild group and the moderate group （P 〈 0.01 ）. But the expression rates of Bcl - 2 and Bax protein were not correlated with the H. pylori infection. But the expression rate of Bcl - 2 protein in the H. pylori（ ＋ ） group was significantly lower than that in the H. pylori（ - ） group in the moderate group（P 〈 0.01 ）. And the expression rates of Bcl - 2 and Bax protein in the H. pylori（ ＋ ） group were significantly higher than that in the H. pylori（ - ） group in the severe group（P 〈0.01 ）. Conclusions The transformation of the mild to the severe precancerous lesions have relations with the changes in the expressions of Bcl - 2 and Bax protein. H. pylori infection influences the expessions of Bcl - 2 and Bax protein in chronic atrophic gastritis accompa- nied by precancerous lesions, which may be one of the mechanisms of apoptosis of gastric mucosal cells induced by H. pylori.

关 键 词：幽门螺杆菌 BCL-2 BAX 慢性萎缩性胃炎 癌前病变 

分 类 号：R735.2[医药卫生—肿瘤]