聚乙二醇胁迫下茶树叶片的蛋白质组分析  被引量:12

Proteomic Analysis of Tea Leaf under Polyethylene Glycol Stress

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作  者:郭春芳[1,2] 孙云[3] 赖呈纯[3] 张木清[1] 

机构地区:[1]福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州350002 [2]福建教育学院,福建福州350025 [3]福建农林大学园艺植物生物工程研究所,福建福州350002

出  处:《茶叶科学》2009年第2期79-88,共10页Journal of Tea Science

基  金:福建省自然科学基金资助项目(B0510025);福建省教育厅科技计划资助项目(JA05333)

摘  要:应用差异蛋白质组学方法分析了铁观音茶树幼苗在聚乙二醇(Polyethylene Glycol,PEG)胁迫下叶片蛋白质组的变化。对18个蛋白质点在PEG胁迫下的变化特点进行了分析,并对有差异表达的16个蛋白质点进行了质谱分析,共鉴定出14个蛋白质点,代表了10种不同的蛋白,其中5个点是同一个蛋白(1,5-二磷酸核酮糖羧化酶/加氧酶大亚基,RubisoLSU),另9个蛋白是PEG胁迫响应蛋白,包括光合系统II23kDa多肽、叶绿体伴侣蛋白21(ch-Cpn21)、磷酸丙糖异构酶(TPI)、三磷酸腺苷合酶β亚基、S-腺苷甲硫氨酸合成酶(SAMS1)、磷脂酰乙醇胺结合蛋白(CEN)、新生多肽相关复合物α亚基(α-NAC)、20S蛋白酶体α亚基(PAE2)、翻译起始因子5A(eIF5A)等,这些蛋白质分别参与了叶绿体组成、糖代谢、能量代谢、硫代谢、蛋白质代谢、信号转导、基因表达调节和细胞程序性死亡等生命活动过程。Two-dimensional electrophoresis(2-DE) were carried out to study the proteomic change in of tea leaves in responsive to polyethylene glycol(PEG) stress, and expression profiles of 18 different proteins were analyzed under PEG stress. Sixteen different proteins were identified by MS/MS using 4700 Proteomics Analyzer(Applied Biosystems, USA),and 14 proteins were identified and represented 10 kinds of proteins. In these proteins, 5 were the same protein--ribulose 1, 5-bisphosphate carboxylase/oxygenase large subunit(RubisoLSU), other 9 proteins were response proteins to PEG stress including photosystem II 23 kDa polypeptide,chloroplast chaperonin 21, triose phosphate isomerase(TPI), ATP synthase subunit, S-adenosylmethionine synthetase I(SAMS1), centroradialis(CEN), nascent polypeptide associated complex alpha chain(α-NAC), 20 S proteasome alpha subunit E2(PAE2) and translation initiation factor 5A(eIF5A). These proteins will play an important role in many life activities of plant, such as chloroplast composition, sugar metabolism, energy metabolism, sulfur metabolism, protein metabolism, signal transduction, gene expression regulation and programmed cell death.

关 键 词:茶树 聚乙二醇胁迫 蛋白质组 双向电泳 质谱 

分 类 号:S571.1[农业科学—茶叶生产加工] Q51[农业科学—作物学]

 

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