微生物絮凝剂产生菌的培养条件优化及其应用  

Optimization and its application of cultural conditions for bioflocculant-producing strain

作　　者：陈晓[1] 王丹丹[1] 李栋芸 刘洋[1] 刘林辉[1] 胡永红[1] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009 [2]南京新百药业有限公司,江苏南京210038

出　　处：《南京工业大学学报（自然科学版）》2009年第2期20-24,共5页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：江苏省高校自然科学基础研究基金资助项目(07KJB530046)

摘　　要：采用正交试验法对微生物絮凝剂产生菌Bacillus subtilisB-3的发酵培养基进行优化.通过对实验结果的统计学分析,得到培养基最佳C源、N源、无机盐质量浓度比为10∶5∶1.在此基础上,对发酵条件进行优化,结果表明:该菌产絮凝剂的最佳培养温度为35℃,初始pH为8.0,转速为200 r/min,培养时间为16 h.用絮凝剂处理不同来源的污水,实验表明,该絮凝剂对南京炼油厂污水、校园生活污水以及南京金川河内污水均具有较好絮凝效果.Using orthogonal experiments, the fermentation medium and cultivation conditions of Bacillus subtilis B-3 were investigated. From the statistical analysis of experimental results, the best mass concentration ratio of carbon resource, nitrogen resource, and inorganic salt was 10： 5： 1. Based on the result, single factor analysis method was used, the optimum cultural conditions were as follows： initial pH value 8.0, temperature 35 ℃, rotated speed 200 r/min, culture period 16 hours. The flocculant presented a good flocculanting effect on the wastewater of refinery, campus and river. The flocculating effects of the flocculants produced by Bacillus subtilis B-3 on the wastewater were studied. The results showed that the flocculent character on the wastewater of refinery, campus and river was obvious.

关 键 词：微生物絮凝剂 正交试验 污水处理 

分 类 号：X172[环境科学与工程—环境科学]