运动对脑老化小鼠铁调节蛋白-2基因表达影响  

Effects of exercise on iron regulatory protein-2 gene expression in brain aging mice

作　　者：云少君[1] 贺晓娟[1] 张淑华[1] 褚金花[1] 肖忠新[1] 魏守刚[1] 

机构地区：[1]首都医科大学公共卫生与家庭医学学院营养与食品卫生学系,北京100069

出　　处：《中国公共卫生》2009年第4期511-512,共2页Chinese Journal of Public Health

基　　金：北京市自然科学基金(7052008)

摘　　要：目的以铁调节蛋白-2(iron regulatory protein-2,IRP-2)基因表达观测点探讨运动抗脑老化的机制。方法将40只雄性ICR小鼠按体重随机分为4组:对照组、脑老化组、运动组、脑老化+运动组。脑老化及运动干预的实验造模期为10周。造模期结束后,以Morris水迷宫试验检测小鼠的学习记忆能力,逆转录-聚合酶链反应(RT-PCR)检测小鼠大脑IRP-2基因表达水平。结果脑老化组小鼠脑IRP-2基因表达水平(0.738±0.221)显著低于对照组(3.938±2.357)(P<0.05);脑老化+运动组小鼠脑IRP-2基因表达水平(2.938±0.468)显著高于脑老化组(P<0.05);与对照组之间差异无统计学意义;运动组IRP-2基因表达水平(2.595±0.822)与对照组之间差异无统计学意义。结论脑老化小鼠IRP-2基因表达水平降低,运动可以防止脑老化小鼠的IRP-2基因表达下调。运动具有抗脑老化的效应。Objective To explore the effects of exercise on the expression of iron regulatory protein-2 （IRP-2） gene in brain aging mice. Methods Forty mice were randomly divided into four groups：control group, exercise group, brain aging group,brain aging ＋ exercise group. The experimental duration was 10 weeks. Morris water maze test was used to measure the learning and memory ability. Reverse transcription polymerase chain reaction （RT-PCR） was used to detect the brain IRP-2 gene expression, Results IRP-2 gene expression decreased in brain aging mice （0. 738 ±0. 221 ） and reached to the extent of statistical significance （ P 〈 0. 05 ）. There was no difference in IRP-2 gene expression between exercise group （2. 595 ± 0. 822） and control group （3. 925 ± 2. 357）. Compared with the control group, there was no remarkable effect in brain aging ＋ exercise group （2. 938 ± 0. 468 ）, but the IRP-2 gene expression in brain aging ＋ exercise group was higher than that in brain aging group （ P 〈 0.05）. Conclusion Exercise can prevent the decreasing of IRP-2 gene expression in brain aging mice.

关 键 词：脑老化 运动 铁调节蛋白-2(IRP-2) 

分 类 号：Q786[生物学—分子生物学]