麦秆分步糖化发酵产乙醇的初步研究  

Preliminary Study on Separate Hydrolysis and Fermentation of Wheat Straw to Ethanol

作　　者：陈玉亮[1] 呼世斌[2] 张守文[1] 王显蕾[1] 赵宇蕾[3] 

机构地区：[1]西北农林科技大学资源与环境工程学院,陕西杨凌712100 [2]西北农林科技大学理学院,陕西杨凌712100 [3]西北农林科技大学生命学院,陕西杨凌712100

出　　处：《西北农业学报》2009年第2期149-153,共5页Acta Agriculturae Boreali-occidentalis Sinica

摘　　要：利用小麦秸秆为原料进行分步糖化发酵产酒精。分别对影响糖化和发酵阶段的各因素进行了研究。结果表明,最适宜的酶解条件为:酶解温度50℃,以pH4.8的醋酸钠缓冲液为溶剂,底物浓度2%,1 mL稀释40倍的纤维素酶液,摇床转速150 r/min;正交试验确定的最佳酒精发酵条件为培养温度30℃,发酵72 h,起始pH4.8,N源补加量为1%,在此条件下当底物浓度为2%时,乙醇的产量可达4.83 g/L。Use wheat straw as raw materials for separate hydrolysis and fermentation（SHF） to ethanol.The factors that impact the hydrolysis and fermentation were studied respectively.The results showed that the best conditions for the enzymatic hydrolysis： temperature 50 ℃,the pH of NaAc-HAc buffer is 4.8,substrate concentration 2%,diluted 40 times cellulose liquid 1 mL,shaker speed 150 r/min,Through orthogonal test found out the optimal ethanol fermentation conditions：culture temperature 30℃,fermentation time 72 h,pH4.8,the additional N-source is 1%.Under the above optimum conditions ethanol concentration reached 4.83 g/L.

关 键 词：纤维素酶 小麦秸秆 乙醇 还原糖 

分 类 号：TQ223.122[化学工程—有机化工]