胃黏膜癌变过程中TFF3、survivin、CD147、CD34表达及其相关性  

Expression of TFF3,survivin,CD147,CD34 in carcinogenesis of gastric mucosa using tissue microarray and their correlation

作　　者：唐忠辉[1] 陈华生[1] 邹宗楷[2] 蔡韶滨[1] 苏海燕[2] 沈洪武[2] 吴文乔[2] 林莉[1] 王元亨[1] 

机构地区：[1]漳州卫生职业学院基础医学部,漳州363000 [2]漳州市医院病理科

出　　处：《山西医科大学学报》2009年第3期202-206,287,共6页Journal of Shanxi Medical University

基　　金：漳州市科技局课题基金资助项目(Z07020)

摘　　要：目的探讨TFF3、survivin、CD147、CD34在不同胃黏膜的表达及其相关性。方法利用组织芯片技术构建包括30例正常胃黏膜和272例患者(浅表性胃炎30例、萎缩性胃炎30例、不典型增生30例及胃癌182例)胃黏膜的组织芯片,同时采用SP免疫组化方法检测TFF3、survivin、CD147、CD34表达。结果萎缩性胃炎、不典型增生和胃癌胃黏膜TFF3表达高于浅表性胃炎和正常对照(P<0.01);胃癌胃黏膜survivin、CD147和CD34表达高于正常胃黏膜、浅表性胃炎、萎缩性胃炎和不典型增生(P<0.05),不典型增生胃黏膜survivin表达高于萎缩性胃炎(P<0.05),浅表性胃炎胃黏膜CD147和CD34表达高于正常胃黏膜(P<0.05)。TFF3表达与胃癌组织类型、淋巴结转移和TNM分期有关(P<0.05),survivin、CD147和CD34表达与胃癌浸润深度、淋巴结转移和TNM分期有关(P<0.05)、CD147表达与胃癌分化程度有关(P<0.01)。TFF3、survivin、CD147阳性表达的MVD高于阴性(P<0.01)。结论TFF3、CD147和CD34可能参与胃黏膜保护及修复,在胃黏膜癌变过程中,TFF3、survivin、CD147、CD34表达逐渐上调,对胃癌发生发展、浸润转移和血管形成有促进作用,可作为早期诊断胃癌和预测其发生转移的重要指标。Objective To investigate expression of TFF3,survivin,CD147,CD34 in carcinogenesis of gastric mucosa,and to explore their correlation. Methods Tissue chip including 30 cases of normal gastric mucosa and 272 cases of gastric mucosa from 30 patients with superficial gastritis,30 patients with atrophic gastritis,30 patients with atypical hyperplasia and 182 patients with gastric carcinoma was prepared using tissue microarray.SP immunohistochemical methods were applied to detect expression of TFF3,survivin,CD147 and CD34. Results TFF3 expression in gastric mucosa of atrophic gastritis,atypical hyperplasia and gastric carcinoma was higher than that in gastric mucosa of normal controls and superficial gastritis（P〈0.01）.Expression of survivin,CD147 and CD34 in gastric mucosa of gastric carcinoma was higher than that in normal controls,superficial gastritis,atrophic gastritis and atypical hyperplasia（P〈0.05）.Survivin expression in atypical hyperplasia was higher than that in atrophic gastritis （P〈0.05）.CD147 and CD34 expression in superficial gastritis was higher than that in normal controls（P〈0.05）.TFF3 was related with histological type,lymph node metastasis and TNM staging（P〈0.05）.Expression of survivin,CD147 and CD34 was related with depth of invasion,lymph node metastasis and TNM staging（P〈0.05）.CD147 was related with tumor differentiation（P〈0.01）.MVD in tissues with positive expression of TFF3,survivin and CD147 was higher than that in negative（P〈0.01）. Conclusion TFF3,CD147 and CD34 may be involved in the protection and restoration of gastric mucosa.During the carcinogenesis of gastric mucosa,TFF3,survivin,CD147 and CD34 may promote the growth,invasion,metastasis and angiogenesis of gastric carcinoma.TFF3,survivin,CD147 and CD34 can be used as important markers for early diagnosis and prediction of metastasis in gastric carcinoma.

关 键 词：胃肿瘤 组织芯片 免疫组织化学 TFF3抗原 survivin抗原 CD147抗原 CD34抗原 

分 类 号：R735.2[医药卫生—肿瘤]