维生素K3增强吉西他滨诱导人膀胱癌T24细胞凋亡的研究  

Effect of vitamin K3 on gemcitabine-induced apoptosis of bladder cancer cell line T24

作　　者：赵亮[1] 刘俊生[1] 

机构地区：[1]山西医科大学第二临床医学院泌尿外科,太原030001

出　　处：《山西医科大学学报》2009年第3期209-211,共3页Journal of Shanxi Medical University

摘　　要：目的探讨维生素K3(VK3)是否能影响吉西他滨诱导人膀胱癌T24细胞凋亡。方法用噻唑蓝(MTT)法筛选出吉西他滨作用T24细胞72h后的适宜作用浓度,再用该浓度联合不同浓度的VK3(2,5,10,20μmol/L)筛选出作用72h后的适宜联合用药浓度。分为三组:空白对照组,吉西他滨组,药物联用组。用PI流式细胞术观察各组细胞凋亡情况,并通过流式软件分析细胞周期变化。结果MTT法筛选出吉西他滨与VK3的适宜药物浓度分别是1.0μg/ml和10μmol/L。药物联用组与吉西他滨组相比,可显著提高细胞抑制率和细胞凋亡率(均P<0.05)。VK3可显著增强吉西他滨诱导细胞集中于S期的作用。结论联用维生素K3可增强吉西他滨诱导人膀胱癌T24细胞凋亡的作用,可能与细胞周期S期阻滞增高有关。Objective To investigate the effect of VK3 on the gemcitabine-induced apoptosis of bladder cancer cell line T24. Methods MTT was used to select the suitable concentration of gemcitabine and the suitable combination concentration of gemcitabine and VK3 for inhibiting T24 cell growth after 72 h culture.Then the cultured T24 cells were allocated into three groups：control group,gemcitabine group and combination group.The apoptosis was detected by flow cytometry using PI staining,and the cell cycle was analyzed by flow cytometry analysis software. Results The suitable concentrations of gemcitabine and VK3 were 1.0 μg/ml and 10 μmol/L,respectively.The inhibitory rate and apoptosis rate in combination group were both higher than in gemcitabine group（P〈0.05）.The arrest of cell cycle in S phase was enhanced in combination group. Conclusion Combination of gemcitabine with VK3 could increase the apoptosis rate of T24 cells,which may be related to its influence on the arrest of cell cycle in S phase.

关 键 词：维生素K3 吉西他滨 T24细胞 细胞凋亡 

分 类 号：R737.14[医药卫生—肿瘤]