短小芽孢杆菌USTB-06发酵液治愈大白鼠烧伤的研究  

The research of Bacillus pumilus USTB-06 broth on burn wound healing in rats

作　　者：关珊珊[1,2] 杨晓静[1,2] 吴鹏飞[1] 闫震[1] 闫海[1] 

机构地区：[1]北京科技大学应用科学学院生物科学与技术系,北京100083 [2]北京科技大学土木与环境工程学院生物化工系

出　　处：《医学动物防制》2009年第4期241-243,F0003,F0004,共5页Journal of Medical Pest Control

基　　金：国家自然科学基金资助项目(20777008);北京市教委共建项目

摘　　要：目的在成功筛选出一株具备很强抑制金黄色葡萄球菌和绿脓杆菌生长的短小芽孢杆菌(USTB-06)基础上,重点在其发酵液对白鼠烧伤模型的治疗效果进行了研究,取得了可以治愈大白鼠烧伤的良好效果。方法16S rDNA基因PCR扩增产物序列分析鉴定筛选菌种,抑菌试验和白鼠烧伤模型试验。结果鉴定筛选菌为短小芽孢杆菌(Bacillus pumilus USTB-06);采用短小芽孢杆菌发酵液直接喷洒于大白鼠烧伤伤口表面,可以明显加快创面的愈合。结论筛选的菌种为短小芽孢杆菌,其发酵液不仅可以明显抑制金黄色葡萄球菌和绿脓杆菌的生长,而且可以促进大白鼠Ⅱ度烧伤创面愈合,具有重要的应用开发前景。Objective Based on the isolation of a promising bacterial strain of Bacillus pumilus （USTB -06） that has a strong ability to inhibite the growth of both Staphylococcus aureus and Pseudomonas aemginosa, the fermentation broth of Bacillus pumilus USTB - 06 for the cure of skin burn in white rats was studied and good results were obtained. Methods 16S rDNA gene sequence analysis of PCR amplification was used to identify an isolated bacterial strain. Inhibition of bacterial growth and the model of the deep second - degree burn of white rats were also used in the experi- ments. Results A very promising bacterial strain was identified as Bacillus pumilus （ USTB -06） on the basis of 16S rDNA analysis with Blast, which can inhibite the growth of Staphylococcus aureus and Pseudomonas aeruginosa. The fermentation broth of Bacillus pumilus （USTB -06） can apparently cure the skin bum in white rats. Conclusion An isolated bacterial strain is identified as Bacillus pumilus USTB -06 and its fermentation broth has a strong ability to inhibite the growth of Staphylococcus aureus and Pseudomonas aeruginosa and cure the the deep second - degree burn wound of white rats, which is very important in application in future.

关 键 词：短小芽孢杆菌 大白鼠 烧伤 治愈 

分 类 号：R-332[医药卫生] R644