大蹼铃蟾皮肤白蛋白的分离纯化及抗体制备  

Purification and Antibody Preparation of Skin Albumin from Bombina maxima

作　　者：张英霞[1] 满初日嘎[2] 张云[3] 

机构地区：[1]海南大学海洋学院,海南海口570228 [2]海南大学生命科学与农学院,海南海口570228 [3]中国科学院昆明动物研究所,云南昆明650223

出　　处：《内蒙古民族大学学报（自然科学版）》2009年第1期44-47,共4页Journal of Inner Mongolia Minzu University：Natural Sciences

基　　金：国家自然科学基金项目(30170195;30470380);海南省自然基金项目(80623);海南省教育厅项目(Hj200731)

摘　　要：通过离子交换层析和凝胶过滤层析,在大蹼铃蟾(Bombina maxima)皮肤中得到其白蛋白(BmA-skin).该蛋白具有较强的胰蛋白酶抑制活性.用0.5mg/只BmA-skin作为免疫源免疫大白兔,经三次免疫后,抽取大白兔血液,分离血清,饱和硫酸氨沉淀,获得抗BmA-skin的多克隆抗体.经ELISA方法测定该抗体与大蹼铃蟾白蛋白发生免疫反应,抗体滴度为1:100000.Skin albumin ion-exchange and gel of Bombina maxima （BmA-skin） was obtained through the steps of mixing filtration chromatography. The protein distinctly inhibited trypsin. The purified BmA-skin was used to immunize rabbits to prepare polyclonal antibody with the dosage of 0.5 mg/per rabbit. After immunized three times, the rabbits were anesthetized with ether, then the blood was collected by a cardiac puncture, and precipitate with saturated ammonium sulfate to obtain polyclonal antibody. ELISA data demonstrated that the antibody titer of the serum was up to 1： 100000. The anti BmA-skin polyclonal antibody had a high quality of potency and specificity.

关 键 词：大蹼铃蟾 白蛋白 多克隆抗体 

分 类 号：Q959.53[生物学—动物学]