甘露聚糖酶基因3′端缺失研究  

Primary Study on the Influence of Deleting 3′ Sequence of β-mannanase

作　　者：王溪森[1] 刘正初[1] 李斌[1] 冯湘沅[1] 段盛文[1] 郑科[1] 成莉凤[1] 

机构地区：[1]中国农业科学院麻类研究所,农业部茎纤维生物质与工程微生物重点开放实验室,湖南长沙410205

出　　处：《安徽农业科学》2009年第8期3496-3497,3579,共3页Journal of Anhui Agricultural Sciences

基　　金：国家"863"计划课题(2006AA02Z249)

摘　　要：[目的]初步研究甘露聚糖酶基因3′端对甘露聚糖酶功能的重要性。[方法]以甘露聚糖酶的二级结构为基础,用PCR扩增的方法获得3′端特异性缺失的甘露聚糖酶DNA片段,连接到载体上,表达并鉴定酶的活性。[结果]删除甘露聚糖酶基因3′端246 bp后甘露聚糖酶依然具有酶活性。[结论]甘露聚糖酶基因3′端的部分序列不是酶具有功能所必需的。[ Objective]The paper aimed to study the importance of 3＇ sequence for the function of β-mannanase. [ Method]β-mannanase DNA segement lack of 3＇ sequence was obtained by PCR amplification according to its secondary structure. The DNA was linked to vector for expressing and identifying the activity of β-mannanase. [ Result ] After 246 bp was shortened from the 3＇sequence, the activity of β-mannanase was still available. [ Conclusion] Part of the 3＇ sequence was not inevitable for the function of β-mannanase.

关 键 词：甘露聚糖酶 序列 删除 

分 类 号：S188[农业科学—农业基础科学]