马铃薯离体再生及人工种子研究  

Studies on in vitro regeneration and artificial seeds of potato

作　　者：唐巍[1] 欧阳藩[1] 郭仲琛[2] 

机构地区：[1]中国科学院化工冶金研究所生化工程国家重点实验室 [2]中国科学院植物研究所

出　　处：《广西植物》1998年第1期65-69,共5页Guihaia

基　　金：国家"863"课题资助

摘　　要：马铃薯３个品种虎头，克４和Ｆａｖｏｒｉｔａ的茎叶外植体在ＭＳ＋１ｍｇ／ＬＮＡＡ＋１ｍｇ／ＬＢＡ培养基上形成愈伤组织。在ＭＳ＋０２ｍｇ／ＬＮＡＡ＋１ｍｇ／ＬＢＡ培养基上，愈伤组织分化产生不定芽。在ＭＳ＋００５ｍｇ／ＬＮＡＡ培养基上，不定芽生根形成再生完整植株。０２～０３ｃｍ大小的不定芽反复继代可持续增殖。有３个以上叶片的不定芽在ＭＳ＋５ｍｇ／ＬＢＡ＋００５ｍｇ／ＬＩＢＡ培养基上和黑暗条件下，在侧芽或顶芽部位形成微型薯。用４％海藻酸钠和２％氯化钙溶液包裹０２～０３ｃｍ大小的不定芽或直径为０２～０３ｃｍ的微型薯制成微芽人工种子和微薯人工种子。在４℃下贮存２个月后，微芽人工种子和微薯人工种子在有菌腐殖土壤中播种２１ｄ的萌发率分别是１５７％和９６２％。Calli of three genotypes of potato of Hutou, Kesi, and Favorita were induced by culturing their stem and leaf explants on MS medium containing 1 mg/L NAA and 1 mg/L BA Adventitious buds were induced from calli on MS medium with 0 2 mg/L NAA and 1 mg/L BA Adventitious shoots rooted to form whole regeneration plants on MS medium suppled with 0 05 mg/L NAA Proliferation of shoots could be obtained on differentiation medium by subcultured of buds of 0 2～0 3 cm in height Microtubers were induced from shoots on MS medium containging 5 mg/L BA and 0 05 mg/L IBA in darkness Artificial seeds were produced by encapsulated both adventitious buds and microtubers of 0 2～0 3 cm in size in 4% sodium alginate and 2% CaCl 2 After preservation at 4 ℃ for two months, the highest germination frequency of bud artificial seeds and microtuber artificial seeds were 15 7% and 96 2% in the 21th day of germination in soil, respectively

关 键 词：马铃薯 离体再生 人工种子 

分 类 号：S532.035.3[农业科学—作物学]