脑星形胶质瘤基因表达及其与临床病理的关系  

Gene Expression and Correlation with Clinical Pathology in Human Gliomas

作　　者：谢季[1] 李代强[1] 姚小明[1] 陈兵[1] 阳楚雄[1] 胡守兴[1] 

机构地区：[1]湖南医科大学附属二院

出　　处：《生命科学研究》1998年第1期68-71,共4页Life Science Research

摘　　要：采用原癌基因Ｂｃｌ－２、Ｐ２１及抑癌基因Ｐ５３、Ｒｂ蛋白４种抗体，用ＡＢＣ免疫组织化学方法对４０例脑星形胶质瘤组织作免疫组化染色．结果发现：Ｂｃｌ－２及Ｐ５３基因表达在Ⅰ～Ⅳ级胶质瘤中阳性率有显著性差异，而且染色强度也有明显差异．Ｐ２１、Ｒｂ基因表达在Ⅰ～Ⅳ级胶质瘤中呈上升趋势，但仅在Ⅰ～Ⅲ级、Ⅰ～Ⅳ级之间有明显差异．可以认为：Ｂｃｌ－２、Ｐ５３、Ｐ２１、Ｒｂ在胶质瘤的发生中发挥一定的作用，尤其是Ｂｃｌ－２、Ｐ５３基因，这些基因蛋白的表达与胶质瘤恶性程度有关．测定胶质瘤中的Ｂｃｌ－２、Ｐ５３、Ｐ２１、Ｒｂ蛋白对于胶质瘤的诊断、鉴别诊断以及判断肿瘤的恶性程度与预后均有一定意义．Immunohistochemical staining method(ABC method) is used to detect the Bcl 2,P53,P21 and Rb gene protein in 40 cases of primary brain gliomas and 5 cases brain tissue.Results showed:the positive rates of Bcl 2 protein,P53 protein expression were remarkably different in I to Ⅳ stage of brain gliomas.The expression of P21 protein,Rb protein was gradually increased from Ⅰ to Ⅳ stage of brain gliomas.Therefore our data indicated the Bcl 2,P53,P21 and Rb gene were important in arising of gliomas,particularly Bcl 2,P53 gene.The expression of these gene were correlated with the degree of malignant of gliomas.Detection of expression of Bcl 2,P53,P21 and Rb gene may be a useful tool for diagnosis,distinguish diagnosis and asserted of malignant and prognosis.

关 键 词：胶质瘤 基因 病理学 脑肿瘤 

分 类 号：R739.410.2[医药卫生—肿瘤]