影响白血病细胞株 K_(562) 对 LDL-ACM 复合物摄取因素的研究  

INFLUENCE THE FACTORS FOR LDLACM COMPLEX TAKE IN LEUKEMIA CELL LINE K562

作　　者：李蔚[1] 胡晓燕[1] 毕文祥[1] 于雪艳[1] 田克立[1] 徐松德[1] 

机构地区：[1]山东医科大学生物化学教研室

出　　处：《山东医科大学学报》1998年第1期27-30,共4页Acta Academiae Medicinae Shandong

摘　　要：以低密度脂蛋白（ＬＤＬ）为阿克拉霉素（ＡＣＭ）的载体，以游离ＡＣＭ为对照，体外研究ＬＤＬ－ＡＣＭ复合物对白血病细胞株Ｋ５６２的细胞毒作用及其影响因素。结果显示：（１）Ｋ５６２细胞对ＬＤＬ－ＡＣＭ复合物摄取较游离ＡＣＭ明显增加，在前３０ｍｉｎ摄取较快，随时间延长摄取逐渐减慢；（２）过量的天然ＬＤＬ、肝素及低温均可使细胞摄取复合物明显减少，而过量的ＨＤＬ则无影响，去脂蛋白血清预刺激的细胞摄取复合物明显增多；（３）对Ｋ５６２细胞的细胞毒作用随复合物浓度增加而逐渐增强，达１０ｍｇ／Ｌ以上时，增加药物浓度其细胞毒作用不再加强。This study was to investigate the feasibility and effetiveness of LDL particles as a carrier for the targeted delivery of a lipophilic anthracycline drug aclacinomycin(ACM) to a chronic myelogenous leukemia in blastic crisis cell line K562.It was found that(1)the time course for LDLACM complex accumulation in K562 cells showed that its cellular uptake was rapid during the first 30 min followed by a slower plase;(2)the accumulation of LDLACM complex was greater than that of free ACM.In the cells drug accumulation was reduced when K562 cells were incubated in the presence of either of an excess of native LDL or heparin,incubated at 4℃.When cells were stimulated by LPDS,cellular drug accumulation obviously increased.But the presence of an excess of native HDL had no effect on cellular drug accumulation;(3)with LDL as a drug carrier,the cell growth inhibition was demonstrated through the experiments of cellular protein concentration,3HTdR incorporation test and cellular survival fraction.The results indicated that the LDLACM complex was more obvious than free ACM.Therefore the conclusion can be drawn that LDL may be used as a good carrier for lipophilic antineoplastic drugs.

关 键 词：低密度脂蛋白类 阿克拉霉素 白血病 药物疗法 

分 类 号：R733.705[医药卫生—肿瘤]