原发性肝癌中 HBV X、S、Pre-S、C 基因片段整合与 ras、myc 癌基因表达的关系  

THE INTEGRATION RATE OF HBV X,S,PreS, C GENES IN PRIMARY HEPATOCELLULAR CARCINOMA AND RELATION TO THE EXPRESSION OF RAS AND MYC ONCOGENES

作　　者：张利宁[1] 刘素侠[1] 孙汶生[1] 曹英林[1] 宋静[1] 马春红[1] 闫世坤[1] 李华芬[1] 

机构地区：[1]山东医科大学微生物学教研室

出　　处：《山东医科大学学报》1998年第1期36-39,共4页Acta Academiae Medicinae Shandong

基　　金：山东省科委资助项目

摘　　要：用地高辛标记的ＨＢＶ基因组探针检测了６６例肝细胞性肝癌（ＨＣＣ）中ＨＢＶ的存在状态，从中筛选出３２例仅有ＨＢＶＤＮＡ整合的ＨＣＣ作为研究对象，进一步检测了ＨＢＶＸ基因、Ｓ基因、Ｐｒｅ－Ｓ、Ｃ基因ＤＮＡ片段的整合情况，并探讨了ＨＢＶ４种基因亚片段整合与ｒａｓ、ｍｙｃ癌基因表达的关系。结果显示，ＨＢＶＸ、Ｓ、Ｐｒｅ－Ｓ和Ｃ基因片段的整合率分别为８７．５％（２８／３２）、６２．５％（２０／３２）、６２．５％（２０／３２）和２５．０％（８／３２）。ｐ２１ｒａｓ、ｐ６２ｍｙｃ在ＨＣＣ中的表达率分别为５０．０％（２０／４０）、８１．２％（２６／３２）。通过对ＨＣＣ中４种ＨＢＶ基因片段整合与ｐ２１ｒａｓ、ｐ６２ｍｙｃ表达之间关系的研究，显示ｐ６２ｍｙｃ的表达与ＨＢＶＸ、Ｐｒｅ－Ｓ基因整合关系密切，ｐ２１ｒａｓ的表达则主要与ＨＢＶＸ基因整合有关（Ｐ＜０．０５）。提示ＨＢＶＸ、Ｐｒｅ－ＳＤＮＡ片段在宿主细胞染色体上的整合可能直接或通过其蛋白启动ｍｙｃ和（或）ｒａｓ癌基因的表达。The existing status of HBV in 66 cases HCC was detected by digoxigenin labelled HBV DNA probe.The 32 cases,with only integrated HBV DNA,were selected and used to study the integration rate of HBV X,HBV S,HBV PreS and HBV C genes and the expression of ras and myc oncogenes.The results showed that the integration rates of HBV X,HBV S,HBV PreS and HBV C genes were 87.5%(28/32),62.5%(20/32),62.5%(20/32),25%(8/32) respectively.The HBV X gene had the obvious higher integration rate than that of other HBV genes(P<0.05).The expression rates of p21ras and p62myc in HCC were 50%(20/40),81.25%(26/32)respectively.The analysis of relation between the integration rate of HBV four genes and expression of p21ras and p62myc showed there was a significant relationship between the expression of myc and the integration of HBV X and HBV PreS genes and also between the expression of ras and the integration of HBV X gene but not in the others.These suggested that the integration of HBV X gene and PreS gene with cellular chromosome may affect the expression of myc and ras oncogenes.

关 键 词：肝肿瘤 乙型肝炎病毒 致癌基因 免疫组化 

分 类 号：R735.702[医药卫生—肿瘤]