抗人膀胱癌基因工程单链抗体的制备研究  

Preparation of the phage single chain antibody of antihuman bladder cancer

作　　者：王元利[1,2] 张宏斌[1,2] 王捷[1,2] 杨太成 江悦华[1,2] 于茂生 

机构地区：[1]广州军区广州总医院泌尿外科 [2]第四军医大学西京医院泌尿外科

出　　处：《中华泌尿外科杂志》1998年第3期131-134,共4页Chinese Journal of Urology

基　　金：国家自然科学基金

摘　　要：应用ＰＣＲ方法，从分泌鼠抗人膀胱癌抗体的杂交瘤细胞中扩增抗体重、轻链可变区基因，经ＤＮＡ接头连接后与噬菌粒ｐＣＡＮＴＡＢ５重组，转化大肠杆菌后，通过亲和筛选、再转染、克隆及鉴定，以此获得抗人膀胱癌噬菌体单链抗体。Using polymerase chain reaction, the immunoglobulin variable region genes were amplified from antihuman bladder cancer hybridomas cells. The heavy and light chain DNA products were assembled into a single gene using a DNA linker. The antibladder cancer single chain DNA fragment was ligated into a phagemid vector pCANTAB5, and the recombinant vector was subsequently introduced into E. coli TG1 cells for construction of phage display libraries of antihuman bladder cancer. By affinity selection, the antihuman bladder cancer single chain antibody of defined specificity and affinity was obtained.

关 键 词：膀胱肿瘤 单链抗体 基因工程 制备 

分 类 号：R392－33[医药卫生—免疫学] R737.140.5[医药卫生—基础医学]