P物质对人多形核白细胞功能的影响  

Effect of substance P on the functions of human polymorphonuclear neutrophilic leucocytes

作　　者：周武庆[1] 法祥光[1] 姜新 卢士红 

机构地区：[1]中国医学科学院中国协和医科大学皮肤病研究所

出　　处：《中华微生物学和免疫学杂志》1998年第2期124-127,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的研究Ｐ物质（ＳＰ）对人多形核白细胞（ＰＭＮ）有关方面作用及可能的意义。方法应用硝基四氮唑蓝（ＮＢＴ）还原法，荧光法和Ｇｒｉｅｓｓ反应等测定不同浓度ＳＰ单独或在细菌衍生肽类似物ＦＭＬＰ甲酯存在下对ＰＭＮ产生超氧阴离子（Ｏ－２，）过氧化氢（Ｈ２Ｏ２），一氧化氮（ＮＯ）和ＰＭＮ膜上一功能酶中性内肽酶（ＮＥＰ）活性及膜流动性的影响。结果ＳＰ（≥１０－５ｍｏｌ／Ｌ）可剌激ＰＭＮ显著产生Ｏ－２，Ｈ２Ｏ２和ＮＯ，后者被Ｌ－单甲基精氨酸（ＮＭＭＡ）所抑制；ＳＰ（１０－８～１０－４ｍｏｌ／Ｌ）能显著增加ＦＭＬＰ甲酯剌激ＰＭＮ产生Ｈ２Ｏ２，并显浓度递增依赖性；ＳＰ与ＦＭＬＰ甲酯协同可下调ＮＥＰ活性；ＳＰ能提高ＰＭＮ膜流动性。结论ＳＰ可通过ＰＭＮ介导调节炎症反应，对ＰＭＮ的杀菌功能有增强作用，ＳＰ对ＰＭＮ的这些影响可能是神经系统参与炎症和免疫调节的途径之一。The neuropeptide substance P(SP) might affect some of the functions of polymorphonuclear neutrophilic leucocytes (PMN) involving inflammation and immunity regulation.In this study, we determined that SP increased the production of O - 2,H 2O 2 and NO, and the activity of a functional enzyme neutral endopeptidas(NEP) and the membrance fluidity of human PMN with or without the presence of bacterial chemoattractant FMLP methyl. The results showed that SP(≥10 5 mol/L) alone could stimulate PMN to produce O - 2, H 2O 2 and NO, which was inhibited by N monomethyl L arginine;SP(10 6 ～10 4 mol/L)promoted H 2O 2 production of PMN in response to FMLP methyl in a dose dependent manner,but downregulated the NEP activity synergied with FMLP methyl; SP promoted the membrance fluidity of PMN. These data indicated that SP could modulate inflammation by PMN and potentiate the antimicrobial function of PMN.Futhermore, SP affecting PMN functions might be a pathway by which nerve system regulated inflammatory and immune reactions.

关 键 词：P物质 多形核白细胞 炎症 

分 类 号：R364.5[医药卫生—病理学]