急性胰腺炎相关性肺损伤模型的建立  

Establishment of Rats Model of Acute Pancreatitis Associated Lung Injury

作　　者：熊玉霞[1] 周业江[2] 孟宪丽[3] 秦大莲[1] 

机构地区：[1]泸州医学院药理教研室,泸州市646000 [2]泸州医学院附属医院普外科,泸州市646000 [3]成都中医药大学药理教研室,成都市610075

出　　处：《医药论坛杂志》2009年第5期7-8,共2页Journal of Medical Forum

基　　金：四川省卫生厅资助项目(80168)

摘　　要：目的采用5%牛磺胆酸钠逆行胰胆管注射建立急性出血坏死性胰腺炎(AHNP)大鼠模型,动态观察肺组织损伤情况,以评价能否建立有效的急性胰腺炎相关性肺损伤(APALI)动物实验模型。方法SD大鼠随机分成假手术对照组和AHNP模型组,动态观察3,6,12和24h四个时相点的血清淀粉酶、胰腺和肺病理组织学改变、肺组织湿干重比和髓过氧化物酶活性,伊文氏蓝法观察1.5,3和6h三个时相点肺血管通透性。结果血清淀粉酶、肺组织湿干重比和髓过氧化物酶活性在5%牛磺胆酸钠诱导后3h明显增高,并持续到24h,肺组织病理检查发现,牛磺胆酸钠诱导后肺组织就出现了明显的充血、出血、炎症细胞浸润、组织结构破坏等肺组织损伤的病理表现,AHNP大鼠1.5h肺血管通透性就显著增高,且随时间呈显著性增加,各指标与假手术组比较差异有显著性(P<0.05)。结论应用5%牛磺胆酸钠能够成功建立APALI大鼠模型,为进一步研究对APALI的防治作用奠定了基础。Objective To establish a rats model of acute pancreatitis associated lung injury （APALI） by retrograde infusion of 5% sodium taurocholate into the biliopancreatic duct. Methods SD rats were randomly divided into sham operation and acute hemorrhagic and necrotic pancreatitis （AHNP） group. The rats were killed at 3,6,12,24 hours after operation. Serum amylase,wet/dry weight ratio and activity of myeloperoxidase of lung were assated. Histopathological alterations of lung was evaluated. Vascular permea- bility of lung were detected by azovan blue at 1.5,3,6h. Results In the AHNP group,serum amylase ,wet/ dry weight ratio,activity of myeloperoxidase, histopathological alterations and vascular permeability of lung were increasing with time,higher than that of sham operation group at same time （ P 〈 0. 05）. Conclusion Apparent lung injury occurs at 3 hours after pancreatitis induced by retrograde infusion of 5% sodium taurocholate into the biliopancreatic duct in rats,suggesting its value in further studies.

关 键 词：急性胰腺炎 肺损伤 大鼠 模型 

分 类 号：R-332[医药卫生]