GC-MS分析蚕蛹油中脂肪酸的成分  

GCMS analysis of fatty acids composition in silkworm pupa oil

作　　者：路萍[1] 赖炳森[1] 颜小林[1] 王映强[1] 郑成贵[1] 谭亚芳[1] 

机构地区：[1]解放军北京医学高等专科学校生化教研室

出　　处：《中国药学杂志》1998年第3期138-140,共3页Chinese Pharmaceutical Journal

摘　　要：目的：化学法提取的蚕蛹油经甲酯化后，分析其脂肪酸的成分。方法：用气相色谱质谱（ＧＣＭＳ）技术及图谱检索，对蚕蛹油中脂肪酸的成分进行鉴定和分析。结果：蚕蛹油中含９种脂肪酸，其中不饱和脂肪酸占７９．８８％，α亚麻酸的相对含量高达７１．４５％。结论：α亚麻酸具有降血脂、降血压和抗癌等生物功能，本研究为α亚麻酸的应用提供了重要的依据。OBJECTIVE: Silkworm pupa oil was extracted by chemical method. Fatty acid in silkworm pupa oil was saponificated by KOHMeOH and was modified to methyl esters by means of BF3MeOH and was checked with thinlayer chromatography. Fatty acids were analyzed. METHOD: The compositions of fatty acids were determined by GCMS and matched with NIST/EPA/NIH library. RESUTLS: Nine compositions in silkworm pupa oil were characterized and determined. The content of unsaturated fatty acids was 79.88%. The αlinolenic acid content in silkworm pupa oil was nearly 71.45% in its fatty acids. CONCLUSION: The αlinolenic acid may decrease plasma lipid and blood pressure, inhibit cancer. The study may be considered significant source for application of αlinolenic acid.

关 键 词：气相色谱 质谱 蚕蛹 脂肪酸 

分 类 号：R284.1[医药卫生—中药学]