乳腺癌与MCF-7乳腺癌细胞株中SP含量的比较  

Comparison of SP Cells between Breast Cancer Tissues and MCF-T Cells

作　　者：王曦[1] 金从国[2] 邹天宁[1] 张季[1] 王明鑫[2] 

机构地区：[1]昆明医学院第三附属医院乳腺病科,云南昆明650018 [2]昆明医学院第三附属医院肿瘤研究所,云南昆明650018

出　　处：《昆明医学院学报》2009年第3期90-93,共4页Journal of Kunming Medical College

基　　金：昆明医学院研究生创新基金(2007207)

摘　　要：目的鉴定乳腺癌组织、癌旁组织、正常组织与MCF-7细胞系中SP(side population)亚群含量是否有差异.方法制备乳腺癌组织、癌旁组织、正常组织细胞悬液和MCF-7细胞悬液,一组加入Hoechst33342染色,另一组同时加入拮抗剂维拉帕米,通过流式细胞仪测定SP亚群含量.结果SP亚群在癌组织、癌旁组织、正常组织和MCF-7细胞的含量分别为(0.54±0.17)%、(7.23±1.12)%、(8.34±0.96)%和(9.58±1.27)%,且它们之间的SP亚群含量具有差异显著性.结论人乳腺癌组织、癌旁组织、正常组织与MCF-7细胞系中都存在SP亚群且含量不同,能够为肿瘤干细胞的鉴定和提纯提供一定的科学参考价值.Objective To identify whether the percentages of SP cells have differences in breast cancer tissues, tumor-adjacent tissues, normal tissues and MCF-7 cancer cells. Methods After staining with Hoechst 3 3342 dye and Verapamil, the percentages of SP cells were detected in cell suspension from breast cancer tissues, tumor-adjacent tissues, normal tissues and MCF-7 cancer cell line respectively with the FACS method. Results The percentages of SP cells in breast cancer tissues, tumor-adjacent tissues, normal tissues and MCF-7 cancer cell line were （0.54 ＋ 0.17）%, （7.23 ＋ 1.12）%, （8.34 ＋ 0.96）% and （9.58 ＋ 1.27）%, respectively. There were significant differences between them. Conclusions Side population cells express in breast cancer tissues, tumor-adjacent tissues, normal tissues and MCF-7 cancer cell line, and the percentages of SP cells are different. This result can supply the scientific value to identify and purify breast cancer stem cells.

关 键 词：乳腺肿瘤 干细胞 细胞 

分 类 号：R737.9[医药卫生—肿瘤]