温敏性品种雌雄蚁蚕蛋白质双向电泳图谱差异分析  

Differential analysis of two-dimensional gel electrophoresis profiles for the newly-hatched male and female larvae of a temperature-sensitive silkworm (Bombyx mori) variety

作　　者：徐秋云[1] 林健荣[1] 毛立明[1] 孔庆明[1] 钟杨生[1] 王叶元[1] 林碧敏[1] 

机构地区：[1]华南农业大学动物科学学院蚕丝科学系,广州510642

出　　处：《昆虫学报》2009年第3期327-338,共12页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30771634);广东省科技计划项目(2007B020706005)

摘　　要：为探讨温敏性品种限伴雌雄蚁体蛋白组分的差异,对刚孵出的雌(黑色)、雄(赤色)蚁体蛋白用双向电泳的技术和方法进行蛋白组分的比较分析。结果显示:在易溶蛋白中,雄蚁和雌蚁分别检测到269和250个蛋白点,能相互匹配的蛋白点占86.71%,特异蛋白雄蚁有43个,雌蚁有25个。在难溶蛋白中,雄蚁和雌蚁分别检测到370和427个蛋白点,能互相匹配的占80.10%,雄、雌蚁的特异蛋白分别为52个和106个。结果表明雌雄蚁体的蛋白组成存在差异。To investigate the differential expression of protein components in the newly-hatched male and female larvae of a temperature-sensitive silkworm variety, Xianban, we compared the protein components of the newly-hatched female （black） and male （red） larvae by using two-dimensional electrophoresis technology. The results showed that 269 and 250 protein spots were detected in easily dissolvable proteins from the newly-hatched male and female larvae respectively. Comparison analysis showed that 86.71% of the total protein spots were matched between male and female larvae, and 43 and 25 specific proteins were found in male and female larvae respectively. In uneasily dissolvable proteins from the newly-hatched male and female larvae, 370 and 424 protein spots were detected respectively. Comparison analysis showed that 80.10% of the total protein spots were matched between the two groups, and 52 and 106 specific proteins were found in male and female larvae respectively. The results indicated that the protein components of the newly-hatched larvae of the silkworm variety are different between the male and female.

关 键 词：家蚕 蚁体 蛋白组分 双向电泳 差异分析 

分 类 号：S882.6[农业科学—特种经济动物饲养]