姜黄素衍生物FM17对K562细胞增殖影响及其与P210^kcr/abl激活的信号途径关系  

The effect of curcumine derivative FM17 on the proliferation of K562 cells and its association with P210^kcr/abl initiated signal pathway

作　　者：陈云艳[1] 许建华[1] 

机构地区：[1]福建医科大学药学院,福建医科大学临床药理所,福建福州350004

出　　处：《世界肿瘤杂志》2009年第1期17-20,共4页Tumour Journal of the World

基　　金：国家自然科学基金资助项目（编号：30873101）

摘　　要：目的探讨姜黄素衍生物FM17对体外培养的慢性粒细胞白血病（CML）急变期细胞K562细胞增生的影响，及其P210^kcr/abl激活的信号途径的关系。方法四甲基偶氮唑蓝（MTT）法检测姜黄素衍生物不同时间点和不同的浓度对K562细胞增殖的抑制作用。western blot的方法分析姜黄素衍生物FM17不同浓度、不同时间点对P210b刮ab0激活的信号的影响。结果2～8μg／mL浓度的FM17在12h、24h、36h及48h均可明显抑制K562细胞增殖，并在一定范围内呈时间和剂量依赖性；FM17可抑制P210^kcr/abl蛋白的含量，且可下调P210^kcr/abl激活的下游信号分子PKC和P-Erk。结论姜黄素衍生物FM17较姜黄素有更强的抗增殖能力，对P210^kcr/abl及其激活的信号途径也有较强的抑制作用。Objective to study the effect of curcumine derivative FM17 on the proliferation of Bcr-abl（＋） K562 cell line and P210^kcr/abl initiated signal pathway. Methods MTT assay was used to detect the inhibition of the proliferation of K562 cell in different time and different dose. The effect of curcumine ramification with different dose in different time on P210bcr/abl2 initiated signal pathway was detected by western blotting analysis. Results The exposure of 2-8μg/ml curcumine ramification to K562 cell could obviously down-regulate the proliferation of KA62 cell, and it was a dose-and time-dependent relationship to some extent. In addition, it could directly inhibit the expression of p210 protein and down-regulate the downstream protein such as PKC, p-Erk, HSP70 initiated by p210. Conclusions Curcumine ramification has a good effect on the proliferation of K562, even better than curcumine, and might have the multiple inhibitory effect on the p210 initiated signal pathway.

关 键 词：姜黄素衍生物 K562 P210^kcr/abl p-stat5 P-ERK 

分 类 号：R73-34[医药卫生—肿瘤]