γ-射线和高温对人视网膜神经胶质细胞的协同抑制作用  

Synergistic suppression of human retinal glial cell proliferation in culture by γ ray and hyperthermia

作　　者：蔡季平[1] 周韵秋[1] 奚寿增[1] 

机构地区：[1]第二军医大学长征医院眼科

出　　处：《中华眼底病杂志》1998年第1期29-32,共4页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的研究γ－射线和高温对培养的人视网膜神经胶质细胞的作用；探索γ－射线和高温联合应用治疗增殖性玻璃体视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅｖｉｔｒｅｏｒｅｔｉｎｏｐａｔｈｙ，ＰＶＲ）的可能性。方法对培养的人视网膜神经胶质细胞进行γ－射线照射、高温处理及两者联用，用ＭＴＴ法测量细胞增殖情况。结果１００ｃＧｙ和３００ｃＧＹ的γ－射线照射量不能有效抑制人视网膜神经胶质细胞，经４２℃或４３℃热处理３０分钟的培养细胞也不被明显抑制。但３００ｃＧｙ照射量联合４２℃热处理３０分钟，培养细胞为对照组的２５．２％，３００ｃＧｙ和４３℃热处理３０分钟则效果更明显。结论较低剂量的γ－射线辐射量联合中等高温处理可抑制培养的人视网膜神经胶质细胞，两者有协同作用。这为在治疗增殖性玻璃体视网膜病变中降低辐射剂量提供依据。Objective To study combination effects of γ ray radiation and hyperthermia on the in vitro cell proliferation of cultured human retinal glial cells in order to explore possible application of the combination treatment for proliferative vitreoretinopathy. Methods Cultured human retinal glial cells were treated by radiation,hyperthermia,or a combination of the two.Cell proliferation was evaluated by MTT method. Results γ ray irradiation of 100cGy or 300cGy was not effective in suppressing proliferation of the retinal glial cells,neither was the heat treatment at 42℃ or 43℃ for 30 min.However,combination of hyperthermia at 42℃ for 30min with 300cGy irradiation suppressed cellular growth of the retinal glial cells to 25.2% of the control.Combination treatment of 43℃,30 min hyperthermia and 300cGy irradiation was more effective. Conclusion A combination of low dose radiation and mild hyperthermia is effective in the suppression of growth of cultured human glial cells,and the effects were found to be synergistic.It is expected that the synergistic effects will lower the radiation dose and also reduce the possible side effects of radiation in the treatment of proliferative vitreoretinopathy.

关 键 词：γ-射线 神经胶质 高温 视网膜病变 

分 类 号：R774.12[医药卫生—眼科]