基因工程菌漆酶降解2-氯苯酚的研究  

Studies on the degradation of 2-chlomphenol by laccase-secreting engineered strain

作　　者：郭梅[1] 路福平[2] 刘敏尧 李托平[1] 梁鹏[1] 蒲军[2] 

机构地区：[1]天津农学院食品科学系,天津300384 [2]天津科技大学生物工程学院工业微生物重点实验室,天津300222 [3]天津诺奥科技发展有限公司,天津300384

出　　处：《河北农业大学学报》2009年第2期61-65,共5页Journal of Hebei Agricultural University

基　　金：天津市自然科学基金重点项目(07JCZDJC03100)

摘　　要：利用基因工程菌甲醇毕赤酵母(PMAD16/pMET(A-Lcc1)培养产生的重组漆酶,研究了重组漆酶对降解2-氯苯酚的影响。结果表明:重组漆酶对2-氯苯酚降解的最佳pH值为6.0,温度为50℃,当溶液中漆酶活力为1.2 U/mL时,在最适脱色条件下作用6 h,粗漆酶和纯化漆酶对2-氯苯酚(250 mg/L)的降解率分别为91.3%和88.2%,重组漆酶可有效地降解2-氯苯酚。The impacts of the recombinant laccase from gene engineered strain on 2- chlomphenol degradation were studied. Results showed that the optimal degradation pH and temperature were pH 6.0 and 500C respectively. Under the optimal conditions, the crude and purified laccase degradation for 250 mg/L 2 - chlomphenol after incubation 6 h with 1.2 U/mL of laccase added were 91.3% and 88.2% respectively. The recombinant laccase could efficiently degrade 2 - chlomphenol.

关 键 词：基因工程菌 重组漆酶 2-氯苯酚 降解 

分 类 号：Q55[生物学—生物化学]