藏黄牛β-乳球蛋白基因启动子部分序列特征  

Characteristics of Partial Sequence of β-lactoglobulin Promoter of Tibetan Yellow Cattle

作　　者：谢双[1] 卢帮敏[1] 金素钰[1] 李玉萍 程瑜 郑玉才[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]四川省九龙县农牧局,四川九龙622000

出　　处：《生物技术》2012年第3期1-4,共4页Biotechnology

基　　金：四川省应用基础课题(No.06JY-0289);高等学校动物科学专业建设点项目(No.TS11126)~~

摘　　要：目的:阐明藏黄牛、牦牛、中国荷斯坦牛β-乳球蛋白(β-Lg)遗传变异体在乳中表达差异的分子基础。方法:采用聚丙烯酰胺凝胶电泳确定乳中β-Lg遗传变异体及其相对表达量,然后利用PCR方法扩增β-Lg启动子部分序列(375bp)并直接测序。结果:在杂合型β-Lg个体中,中国荷斯坦牛乳中β-Lg A的相对表达量(63.7±2.9%)均一致性地高于β-Lg B,而藏黄牛乳中二者比例十分接近,但个体差异差异较大。16个测序样品中共检测到13处碱基突变,其中有5处为牦牛特异的。另外,在牦牛β-Lg启动子-452与-453之间,还存在一个插入碱基。结论:β-Lg启动子-430碱基在中国荷斯坦牛中表现为等位基因特异的,而在藏黄牛中无等位基因特异性。Objective:The objective of present study was to reveal the molecular basis for the differential expression of β-lactoglobulin(β-Lg) in milks of Tibetan Yellow cattle,yak and Chinese Holstein.Method:Polyacrylamide gel electrophoresis was used to determine the genetic variants of β-Lg and their relative amounts in milk,and partial sequence(375bp) of β-Lg promoter was amplified by PCR and direct sequencing.Result:The relative percentage of β-Lg A in the milk(63.7±2.9%) was higher than β-Lg B in Chinese Holstein carrying heterogous β-Lg,while in the milk of Tibetan Yellow cattle,their relative amounts were similar,with higher variations among individuals.A total of 13 base mutations were detected among the 16 β-Lg promoter sequences assayed,five of which were specific for yaks.Furthermore,a base insertion was observed between-452 and-453 of yak β-Lg promoter.Conclusion:The base mutation at-430 of β-Lg promoter showed allele-specific in Chinese Holstein,but not in Tibetan Yellow cattle.

关 键 词：藏黄牛 牦牛 Β-乳球蛋白 启动子 基因表达 

分 类 号：S823[农业科学—畜牧学]