抗人肝癌抗体导向的载药毫微粒在荷瘤裸鼠体内的分布  

Distribution of human hepatoma-specific antibody-directed immunonanoparticles in tumor-bearing nude mice

作　　者：肖笑荣 刘晓波[1] 胡质毅[2] 蔡美英[3] 张萃[1] 

机构地区：[1]广东药学院微生物学与免疫学教研室,广州510006 [2]广州中医药大学国家重点学科实验室 [3]四川大学基础医学与法医学院免疫学教研室

出　　处：《中华临床医师杂志（电子版）》2012年第24期8147-8150,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：教育部回国留学人员启动基金(44143006);卫生部科研基金(98-1-225)

摘　　要：目的观察抗体导向免疫毫微粒在荷瘤裸鼠体内的分布。方法采用氯胺T法将131I核素标记到人肝癌特异性抗体HAb18导向的阿霉素白蛋白免疫毫微粒;于皮下荷人肝癌SMMC-7721裸鼠模型体内,分别经尾静脉、腹腔或瘤体注射131I标记的免疫毫微粒,一定时间后处死裸鼠,取肿瘤、血、心、肝等组织,计算免疫毫微粒在各组织中的分布量。结果经腹腔或尾静脉给药,免疫毫微粒主要分布于肝、脾等处,而在异位肿瘤处分布很少;经瘤体给药,免疫毫微粒主要分布于肿瘤处,在其他部位分布极少;比较肝癌特异性抗体免疫毫微粒、对照抗体免疫毫微粒及普通毫微粒在肿瘤中的分布,第12天后,肝癌特异性抗体免疫毫微粒的肿瘤滞留量明显高于其他两种毫微粒。结论抗体导向的载药免疫毫微粒经腹腔、尾静脉给药无主动靶向结合异位肿瘤作用;但经瘤体给药能较好地滞留在肿瘤中。Objective To observe the in vivo distribution of antibody-directed immunonanoparticles in tumor-bearing nude mice and then determine the optimal in vivo administered route of the immunonanoparticles.Methods Ammonia chroloric T technique was used for coupling radioisotope 131I to adrimycin-loaded human serum albumin immunonanoparticles linked with human hepatoma specific antibody HAb18.The nude mice bearing human hepatoma xenograft subcutaneously were administered with labeled-immunonanoparticles by tail vein,peritoneal cavity or intratumor route.The mice were sacrificed at the appointed time,their tumors,bloods,hearts etc.were weighted and then the distributive amounts of the labeled-immunonanoparticles in these tissues were calculated.Results Following administration by tail vein or peritoneal cavity route,immunonanoparticles mainly concentrated in liver,spleen,etc,but few in the tumor xenografts.However,following administration by intratumor route,immunonanoparticles mostly remained in tumor xenografts and had significantly higher remaining amount than control immunonanoparticles after 12 days of administration,which suggested the key role of the tumor-specific antibodies in the retardation of immuoparticles within tumor.Conclusions Immunonanoparticles couldn't actively transfer to heterotopic tumor xenograft when administered by tail vein or peritoneal cavity route,but had much higher remaining amount in tumor when administered by intratumor route.

关 键 词：抗体 肿瘤 小鼠 纳米粒子 载药毫微粒 

分 类 号：R735.7[医药卫生—肿瘤]