Mineralized and Osteoid Tissue from Dental Pulp Stem Cells on Micro-arc Oxidation Titanium in vitro  

作　　者：黄怡 常婷 杨成 吴梦娟 

机构地区：[1]Department of Stomatology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2012年第4期620-625,共6页华中科技大学学报（医学英德文版）

基　　金：supported by the Innovation Fund of Huazhong University of Science and Technology,Wuhan,P.R. China (No.2011JC018)

摘　　要：The presence of insufficient bone volume affects the implant healing and success.The aim of this study was to evaluate osteogenic capacity of dental pulp stem cells(DPSCs) on micro-arc oxidation(MAO) titanium surface.DPSCs were challenged at MAO and smooth titanium surface separately for different durations,and the bone marrow mesenchymal stem cells(BMSCs) served as the positive controls.The osteogenic capacity of DPSCs on MAO titanium surface was assessed by using scanning electron microscopy,energy dispersive spectroscopy,biochemical tests and real-time quantitative PCR.Data showed that DPSCs differentiated into osteoblasts and expressed bone morphogenetic genes on MAO titanium surface.The results of this study revealed that DPSCs had good potential to generate mineralized tissue on MAO titanium plates.The differential potential of DPSCs may be regulated by MAO titanium surface.The osteogenesis potential of DPSCs on the MAO titanium was similar with BMSCs.The presence of insufficient bone volume affects the implant healing and success.The aim of this study was to evaluate osteogenic capacity of dental pulp stem cells(DPSCs) on micro-arc oxidation(MAO) titanium surface.DPSCs were challenged at MAO and smooth titanium surface separately for different durations,and the bone marrow mesenchymal stem cells(BMSCs) served as the positive controls.The osteogenic capacity of DPSCs on MAO titanium surface was assessed by using scanning electron microscopy,energy dispersive spectroscopy,biochemical tests and real-time quantitative PCR.Data showed that DPSCs differentiated into osteoblasts and expressed bone morphogenetic genes on MAO titanium surface.The results of this study revealed that DPSCs had good potential to generate mineralized tissue on MAO titanium plates.The differential potential of DPSCs may be regulated by MAO titanium surface.The osteogenesis potential of DPSCs on the MAO titanium was similar with BMSCs.

关 键 词：dental pulp stem cells micro-arc oxidation osteogenic differentiation mineralized tissue dental implant 

分 类 号：R780.2[医药卫生—口腔医学]